摘要
目的:探讨18F-脱氧葡萄糖(18F-FDG)单光子发射式计算机断层显像(SPECT-CT)对大鼠肠辐射损伤检测的敏感性。方法:选取20只雄性SD大鼠随机分为两组,即正常组和模型组。模型组大鼠进行60Co一次性全身照射8.0Gy,观察大鼠一般情况;模型组腹泻症状明显时立即做18F-FDGSPECT-CT并检测肠道浓聚影;检测后取出大鼠小肠,4%甲醛固定,常规病理切片,于电子显微镜下观察。结果:(1)6只大鼠照射4d后出现腹泻,3只5d后出现腹泻,1只观察7d未出现腹泻。(2)SPECT-CT显示腹泻大鼠肠道有明显浓聚影,正常组大鼠肠道无明显浓聚影。(3)病理显示:模型组大鼠小肠破坏严重,炎症明显;对照组大鼠小肠无粘连、局部充血,肠壁不增厚,未见溃疡。结论:18F-FDGSPECT-CT作为一种无创性检测方法,能够直观有效地检测大鼠肠辐射损伤。
Objective To study the sensibility of 18F-fluorodeoxyglucose （18F-FDP ） SPECT-CT in rats with radiative injury. Methods Twenty SD rats were randomly divided into two groups：control group （n = 10） and model group （n = 10）. Whole body radiation of 8.0 Gy was performed in model rats. Then observed the state of each rat. 18F-FDG SPECT-CT checked them immediately when diarrhea happened in modle rats,and then these rats were dissected,their intestines were take out for pathological sections. Observed the changes of intestines of model rats by electron microscope. Results （1）diarrhea were found in the model group,six rats happened 4 days after the radiation,and three rats happened in 5 d,while one rat didn＇t have diarrhea till the 7 d. （2）The umbra of intestine showed clear in model rats by SPECT-CT,while did not show in the control rats. （3）General form and observational findings on pathohistology：No adhesion,local congestion,thickening of colon wall,and no ulcer were seen in the control group,while the model rats showed adherence,incrassation,hyperemia,dropsy,ulceration,thickening of intestinal tract,hydropsia of under layer of mucosa,and inflammatory cells infiltration in tunica intima. Conclusion ^18F-FDG SPECT-CT can detect irradiation injury on intestinal tract of rats without harming living organism.
出处
《实用医学杂志》
CAS
北大核心
2010年第4期574-575,共2页
The Journal of Practical Medicine
