摘要
通过相关系数的计算与显著性检验，结合病毒蛋白浓度的大小，同时进行电镜观察，证实了由鸭胚增殖的ＥＤＳ病毒采用６０％饱和度的硫酸铵或１０％聚乙二醇（ＭＷ６０００）沉淀法初提后，再经ＳｅｐｈａｄｅｘＧ－２００层析法提纯的效果优于ＤＥＡＥ５２纤维素色谱法。
Effects of purifying EDSV by 60% (NH 4) 2SO 4 or 10% PEG(MW6000) and then running Sephadex G-200 are better than DEAE 52 's.The results are confirmed by means of coefficient of correlation and t test,in combine with viral total protein concentration and microscopic observation.
出处
《山地农业生物学报》
1998年第5期282-286,共5页
Journal of Mountain Agriculture and Biology
关键词
鸡
鸡减蛋综合征
EDSV
病毒抗原
EDSV coefficient of correlation viral antigen protein concentration Sephadex G-200 DEAE 52
