摘要
用带有酶动力学分析软件的紫外分光光度计,测定多种中药提取物对黄嘌呤氧化酶的抑制活性.测定波长为294 nm,黄嘌呤氧化酶1.14 units/mL.筛选在体外能抑制黄嘌呤氧化酶活性的中药提取物,并比较了它们的抑制活性.结果表明枳椇子乙醇提取物、高良姜乙醇提取物和葛花乙酸乙酯提取物对黄嘌呤氧化酶显示出较强的抑制活性;说明上述3种提取物是抑制黄嘌呤氧化酶的活性部位,可进一步分离纯化得到有效化合物.
Xanthine oxidase inhibitory activity of multiple herbal medicine samples were determined by UV spectrophotometer with enzyme kinetics analysis software, wavelength 294 nm, xanthine oxidase 1.14 units/ mL. The extracts of Chinese herbal medicines were screened, which can inhibit the activity of xanthine oxidase in vitro, and their inhibitory activity were compared. The resules were that ethanol extracts of Semen Hoveniae, ethanol extracts of Rhizoma Alpiniae officinarum and ethylacetate extract of Flos Pueraiae showed some inhibitory activity on xanthine oxidase; and the above-mentioned extracts may be active fraction inhibiting XOD and could get effective compounds by further separation and purification.
出处
《武汉工程大学学报》
CAS
2010年第3期44-46,共3页
Journal of Wuhan Institute of Technology
关键词
枳椇子
高良姜
葛花
黄嘌呤氧化酶
动力学分光光度法
Semen Hoveniae
Rhizoma Alpiniae officinarum
Flos Pueraiae
xanthine oxidase
kinetic spectrophotometry
