摘要
以亚洲百合俄维侬幼嫩花蕾为材料，采用不同浓度秋水仙碱溶液处理幼嫩花蕾来得到染色体加倍的2n花粉。观察花粉母细胞减数分裂过程，探讨其减数分裂过程与花蕾大小的对应关系，确定诱导2n花粉的有效时期。结果表明：花蕾长度为1．0～2．4cm时是2n花粉的有效诱导时期，以0．1％的秋水仙碱溶液连续注射处理3d，效果最佳，诱导率高达33．2％。
The methods to doubled pollen chromosome of Avignon were studied. Using flower buds of Avignon as materials,the meiosis processes of pollen mother cells were observed. In order to know when 2 n pollen could be induced, the relations between flower buds length and pollen mother cells developmental stages were studied. Then it induced doubling pollen chromosome with various concentrations of colchicines in different treatments. The results indicated that the best time for 2 n pollen induction was that the length of flower buds was 1.0-2. 4 cm. The highest percentage of unreduced pollen obtained by treating flower buds for 3 days with 0. 1% colchicines was 33.2%.
出处
《北方园艺》
CAS
北大核心
2010年第6期144-147,共4页
Northern Horticulture
基金
沈阳市科技攻关资助项目(1071146-3-00)
