摘要
目的分析黄芩苷对小鼠肠道微生态的影响。方法40只NIH小鼠,雌雄各半,随机平均分为4组,即生理盐水组、林可霉素组、低剂量黄芩苷组(1.25mg/mL)和高剂量黄芩苷组(2.5mg/mL)。每组小鼠相应灌胃14d后,对各小鼠肠道粪便的细菌群落进行细菌培养分析和PCR-DGGE(PCR-变性梯度凝胶电泳)分析。结果低剂量黄芩苷组的细菌物种丰度、多样性指标以及乳杆菌数目都高于其他各组。生理盐水组、林可霉素组和低剂量黄芩苷组的肠道细菌种群表现为组内相似性较高,组间差异性较大。结论低剂量黄芩苷能促进某些细菌、很可能是乳杆菌的生长;低剂量黄芩苷还可调节肠道形成新的、更稳定的细菌群落结构。
Objective To analyze the influence of baicalin on intestinal microflora in mice. Methods Forty mice （male in half）were divided into four groups： normal saline group, cillimycin group, low-dose baicalin group （1.25 mg/ mL） and high-dose baicalin group（2.5 mg/mL）. Each group with 5 female and 5 male mice was treated with intragas- tric administration of corresponding medicine. PCR-DGGE（PCR-denaturing gradient gel electrophoresis）and bacterio- logical analysis were employed to analyze the intestinal microbial communities of the mice. Results Low-dose baicalin group had highest Shannon-Wiener index, species richness and lactobacilli count in the four groups. Bacterial species in normal saline group, cillimycin group and low-dose baicalin group showed high intragroup similarity and high inter- group diversity. Conclusion Baicalin of low dose could promote the growth of some bacteria such as Lactobacilli and enhance the formation of some new and more stable intestinal microbial communities.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2010年第2期123-126,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广州中医药大学创新基金(09CX007)
