摘要
为了解间二硝基苯（ｍＤＮＢ）对肝细胞是否有遗传毒性，以及胆红素对此是否有保护作用，本研究在体外用单细胞凝胶电泳技术测定了ｍＤＮＢ对肝细胞ＤＮＡ的损伤作用及胆红素的保护作用。结果表明，ｍＤＮＢ可导致肝细胞ＤＮＡ链断裂，且有明显的剂量反应关系，同时加入胆红素可部分抑制ｍＤＮＢ所致的ＤＮＡ损伤，提示ｍＤＮＢ是一种遗传毒物。
In order to study the genotoxicity of m dinitrobenzene (m DNB) in hepatocytes and the protective effects of bilirubin, single cell gel electrophoresis (SCGE) assay was used to detect the DNA strand breaks in hepatocytes exposed to m DNB singly and m DNB+bilirubin The results showed that m DNB could induce DNA strand breaks significantly with obvious dose dependent manner, and bilirubin could inhibit this effect partly when hepatocytes were exposed to m DNB and bilirubin simultaneously, suggesting that m DNB has genotoxicity in hepatocytes and bilirubin may inhibit this genotoxicity effectively
出处
《职业医学》
1998年第6期5-6,共2页
China Occupational Medicine
