摘要
目的观察玄参提取物对大鼠动脉硬化及其炎症相关细胞因子的影响。方法将大鼠随机分为正常组、模型组、玄参提取物低剂量组、玄参提取物高剂量组和布洛芬组。模型组及各给药组采用一次性腹腔注射维生素D360万单位/kg,同时每天灌服10 ml/kg脂肪乳的方法制备大鼠动脉硬化模型。玄参低剂量组和高剂量组分别每天灌服玄参提取物20.5mg.kg-1.d-1和41.0 mg.kg-1.d-1,布洛芬组每天灌服布洛芬混悬液144 mg.kg-1.d-1,正常组和模型组每天灌服等量蒸馏水。给药70 d后处死大鼠,对血脂、TNF-α,IL-1β,IL-6,IL-10及NF-кB进行测定,并观察动脉的病变情况。结果玄参提取物可明显降低模型大鼠胆固醇和低密度脂蛋白的水平,提高高密度脂蛋白与低密度脂蛋白的比值,降低炎症因子TNF-α,IL-1β,IL-6的浓度,提高抗炎因子IL-10的浓度,抑制动脉NF-кB过量表达,抑制模型大鼠动脉壁中膜的增厚。结论玄参提取物可通过抗炎和降血脂而发挥一定的抗动脉硬化的作用。
Objective To study the effect of extract from Radix Scrophulariae（RS） against atherosclerosis and the relative mechanisms in rats.Methods Rats were randomly divided into 5 groups.They were the conrtol,model,low and high dose of RS extract and ibuprofen groups.The model rats were intraperitoneally injected with 600 000 IU·kg^-1 vitamine D3 and intragastrically administrated high-fat emulsion 10 mg·kg^-1·d^-1 for 70 days.At the same time,the model rats treated with drugs were orally given 20.5mg·kg^-1·d^-1,41.0 mg·kg^-1·d^-1 RS extract and 144.0mg·kg^-1·d^-1 ibuprofen suspension respectively.After 70days,the rats were killed,and the serum levels of TC,TG,LDL,HDL/LDL,TNF-α,IL-1β,IL-6,IL-10,the expression of NF-кB in rat aorta and the pathological change of aorta were measured.Results RS extract could lower the serum levels of TC,LDL-C,TNF-α,IL-1β and IL-6,increase the level of IL-10 and the ratio of HDL/LDL,inhibit the expression of NF-кB and reduce the thickness of the artery wall.Conclusion RS extract has anti-atherescle ratic effect.The mechanism may be related with inhibiting inflammation and lowering serum lipid.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第3期532-534,共3页
Lishizhen Medicine and Materia Medica Research
基金
上海市教育委员会科研项目(No.06CZ035)
科技部"重大新药创制"科技重大专项(No.2009ZX09103-398)
关键词
玄参提取物
动脉硬化
抗炎
血脂
Radix Scrophulariae extracts
Atherosclerosis
Anti-inflammation
Serum lipid
