摘要
采用自行设计、组装的毛细管电泳光导纤维发光二极管诱导荧光检测装置，建立了一种直接测定免疫球蛋白G（IgG）的方法。以蓝色发光二极管（LED）为荧光检测器的激发光源，荧光素异硫氰酸酯（FITC）为柱前衍生试剂，采用毛细管区带电泳，以20mmol／L硼砂缓冲溶液（pH9．2）为背景电解液进行分离检测。通过对衍生反应条件和电泳分离条件进行优化，确定了最佳实验条件，在该条件下，IgG的线性范围为4．5×10^-8-1．2×10^-6g／L，检出限为2．0×10^-8g／L。该方法简单、高效、选择性好，无需前处理，可用于人血清中IgG含量的测定。
A new method for direct determination of IgG without purification was developed by caprilary electrophoresis （CE） with optical fiber light-emitting diode （LED） induced fluorescence detection. Fluorescence isothioeyanate （FITC） was selected as precolumn derivatization reagent in capillary zone electrophoresis. The conditions of derivatization and separation were optimized. The result indicated that the best derivatization conditions was used buffer of 10 mmol/L borate, reaction time of 16 h at room temperature, and the best separation conditions was used a background electrolyte solution of 20 mmol/L borate buffer（ pH 9.2） and separation voltage of 18 kV. Under the optimal conditions, the calibration curve was linear in the range of 4. 5 ×10^-8 - 1.2 ×10^-6 g/L. The limit of detection for IgG was 2.0 ×10^-8 g/L. The method showed advantages of simpleness, rapid separation, high sensitivity and without prophase disposal in the system, and could be direct applied in the detection of IgG in human serum sample.
出处
《分析测试学报》
CAS
CSCD
北大核心
2010年第3期257-261,共5页
Journal of Instrumental Analysis
