摘要
通过PCR法从奥利亚罗非鱼DNA中扩增出MSTN基因。该序列全长1 957 bp,含有3个外显子,2个内含子。外显子Ⅰ379 bp,外显子Ⅱ371 bp,部分外显子Ⅲ145 bp,内含子Ⅰ305 bp,内含子Ⅱ751 bp,编码区895 bp,共翻译298个氨基酸。通过随机测序法筛选SNPs,获得了6个突变位点,外显子Ⅰ、Ⅱ各有一个,而内含子Ⅱ则含有4个突变位点。外显子Ⅰ、Ⅱ的突变位点均导致翻译的氨基酸序列发生改变,为错义突变。研究结果为SNPs位点与奥利亚罗非鱼生长性能关联分析奠定了基础。
Oreochromis aureus MSTN was isolated by using PCR technique.The whole sequence length was 1 957 bp including three exons and two introns.The sequence length of exon Ⅰand Ⅱwas 379 bp,371 bp respectively,and partial exon Ⅲ was 145 bp.The sequence length of InronⅠand Ⅱ was 305 bp and 751 bp respectively.The ORF was 895 bp encoding 298 amino acids.Six SNPs was obtained through sequencing MSTN from 10 randomly selected individuals in Oreochromis aureus.Four SNPs were found in the Intron Ⅱ,however,only one SNPs was observed in the Exon I and Ⅱrespectively.The mutation sites in the Exon I and Ⅱchanged for amino acids indicated that they were sense mutation.The SNPs might be useful for studying MSTN gene association to growth trait in Oreochromis aureus.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2010年第2期157-161,共5页
Journal of Shanghai Ocean University
基金
基本科研业务费专项资金(2007JBFC03
2007JBFC04)
国家科技基础条件平台(2007DKA30470_003)
