摘要
目的阐明Ｂ７－１在乙型肝炎肝组织中的表达及其与病变发生、发展的关系。方法用免疫组织化学方法检测连续切片的乙型肝炎肝组织Ｂ７－１及表面抗原表达。结果正常对照２例均为阴性；１７例慢性迁延性肝炎（ＣＰＨ）中，胞浆Ｂ７－１阳性的有１６例，轻度阳性８例，中度５例，重度３例；１４例慢性活动性肝炎（ＣＡＨ）全部阳性，其中轻度阳性２例，中度３例，重度９例，经等级秩和检验，两者差异显著（Ｐ＜００１）。急性黄疸性肝炎、亚急性重型肝炎轻度阳性。在３４例乙型肝炎肝组织中，３３例ＨＢｓＡｇ阳性，轻度阳性３例，其余为中到重度阳性，且ＣＰＨ与ＣＡＨ两组之间无统计学差异。结论（１）乙型肝炎病毒感染可诱导Ｂ７－１在肝组织表达，且表达强弱与肝炎病变程度正相关；（２）乙型肝炎表面抗原在组织中表达强弱与Ｂ７－１表达无关。（３）Ｂ７－１在肝组织中的表达可能是肝细胞活化积极参与免疫反应的佐证。
Objective To investigate the relationship between B 7-1 expression and liver damage of patients with hepatitis B. Methods Consecutive paraffin sections were immunohistochemically stained with anti-B 7-1 and anti-HBs monoclonal antibodies, respectively. Results It was observed that B 7-1 antigen expressed variously in the cytoplasm of hepatocytes. In chronic persistent hepatitis B(CPH) group, B 7-1 expressed negatively in 1, mildly in 8, moderately in 5, and strongly in 3 samples, respectively. In contrast, in chronic active hepatitis B(CAH) group, B 7-1 expressed mildly in 2, moderately in 3 and strongly in 9 samples, respectively. There was statistical difference between the two groups by Wilcoxon's test. One subacute fulminant and 1 acute icteric hepatitis B(AIH) samples expressed mildly. Hepatitis B surface antigen (HBsAg) expressed in 33 of 34 samples with different levels, mostly expressing moderately to markedly and in the cytoplasm of hepatocytes, a few both in the cytoplasm and on the membrane. Conclusion (1) B 7-1 expressed in the liver tissues might be induced by HBV infection and its expression was closely correlated with the activity of viral hepatitis B. (2) There was no direct relationship between B 7-1 expression and the level of HBsAg expression. (3) Like other adhesion molecules, B 7-1 expression in liver tissue with HBV infection was probably one of the evidences that hepatocytes were activated and actively involved in the immune regulation during HBV infection.
出处
《中华肝脏病杂志》
CAS
CSCD
1998年第4期203-205,共3页
Chinese Journal of Hepatology
