摘要
关于生物组织传感器的研究,Arnold有详细综述。刀豆中包含丰富的脲酶,本文报道将刀豆切片与自制石墨内导PVC膜pH电极型氨气敏电极偶合制成了刀豆切片脲电极,对其响应特性、介质条件、固定化及切片厚度等因素进行了研究,取得了较满意的结果。
A jack bean tissue section electrode sensitive to urea has been prepared by coupling a jack bean slice with an ammonia gas-sensing probe using PVC membrane pH electrode as the internal element. The potentiometric response characteristics of this new electrode under steady-state and dynamic condition were examined. The steady-state linear response ranse of this electrode is 1.0 × 10-2-4.6 ×10-5mol/L, with a detection limit of 4.0 × 10-5mol/L. The influence of some experimental parameters including buffer composition, operation pH, etc. are discussed. Urea was determined by using dynamic method, with an average recovery of 98.9% and a standard deviation of 1.8%.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1989年第3期308-310,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
生物传感器
脲
刀豆切片电极
Biosensor, Tissue section electrode, Determination of urea, Jack bean section electrode
