摘要
目的探讨LPS等丝裂原所致THP-1细胞增殖分化的优化因素。方法利用MTT法及改良WST-8法测定THP-1细胞的增殖情况,并对THP-1细胞生长特性及其曲线进行测定,同时测定成熟THP-1细胞的吞噬功能。由此探讨LPS、PHA、ConA对PMA诱导成熟的THP-1细胞活化增殖的最佳浓度及作用时间曲线。结果WST-8法测定THP-1细胞增殖活性优于MTT法,比较敏感;THP-1细胞增殖测定表明LPS比PHA、ConA效果好;LPS浓度在1μg/ml、作用24h可充分诱导活化巨噬细胞,同时巨噬细胞具有吞噬鸡红细胞的能力。结论LPS可使巨噬细胞活化增殖,并且吞噬功能明显增强。
Objective To evaluate the optimization factors of LPS and other mitogens-induced THP-1 cell proliferation. Methods The WST-8 assay,a modification of MTT assay,was used to determine the THP-1 cell proliferation in experimental conditions and the phagocytosis of mature THP-1 cells. The optimal concentration and time of LPS, PHA and ConA inducing the activation of mature THP- 1 cells were investigated, and the proliferation curve was determined. The optimal concentration and time of LPS inducing macrophage were observed. Results WST-8 was better and more sensitive than the MTr method to determine the activation of THP-1 cell proli- feration. The effect of LPS inducing proliferation was better than that of PHA, ConA. The LPS fully induced the activation of macrophage at the concentration of 1 μg/ml for 24 h,and the activated giant macrophage had the phagocytosis for chicken red blood ceils. Conclusion LPS could activate the proliferation of macrophages and increase the phagocytosis.
出处
《山西医科大学学报》
CAS
2010年第3期222-224,共3页
Journal of Shanxi Medical University
基金
广东药学院博士启动基金资助项目(2006JGX08)
