摘要
CpG岛和TATA框在基因表达调控方面具有重要的作用，本研究从EBI核酸数据库中下载经试验证实的果蝇启动子序列共计1917条，对其中的CpG岛、GC碱基分布和TATA框进行了分析。预测果蝇启动子序列中CpG岛的最佳参数组合为：GC最低含量为0．44，CpG最低出现率为0．6，CpG岛最小长度为200bp，在此条件下，发现有84．82％的果蝇启动子中含有1—2个独立的CpG岛。果蝇启动子序列的平均GC含量为39．83％，GC碱基在启动子中的分布表现出规律性变化。果蝇启动子中TATA框的分布比较广泛，有32．86％的启动子中含有1个TATA框；有18．73％的启动子中含有2个TATA框；另有13．88％的启动子中含有2个以上的TATA框；此外，有34．53％的启动子没有TATA框。表明果蝇启动子中有比较丰富的CpG岛和TATA框。
CpG island and TATA-box had important effect in controlling gene transcription.1 917 fruit fly promotor sequences which had been proved by other studies were downloaded from EBI nucleotide database and the distribution of CpG islands,GC proportionality and TATA-box were analyzed by bioinformatics motheds.The optimal sets of combined parameters to predict CpG islands in fruit fly promotors were that the minisland was 200 bp,the GCmin was 0.5 and the GCoe was 0.6.Under this condition,84.82% promotors had one or two CpG islands.The average GC percentage was 39.83 % in fruit fly promotors.The distribution of GC in fruit fly promotors was changed regularly.The TATA-box spreaded widely in fruit fly promotors.About 32.86% promotors had one TATA-box,18.73% promotors had two TATA-boxes and 13.88% promotors had more than two TATA-boxes;the other 34.53% promotors had no TATA-box.This indicated that the CpG island and TATA-box were rich in fruit fly promoters.
出处
《生物信息学》
2010年第1期82-85,90,共5页
Chinese Journal of Bioinformatics
基金
河南科技大学科研启动费
项目编号:09001241
