摘要
目的研究外阴硬化性苔藓患者病变组织中的HLA-DR抗原表达和外周血中T细胞亚群水平的变化,探讨上述因素在其发病中的作用机制。方法应用免疫组化SP法检测30例患者(研究组)及21例其他良性病变患者(对照组)外阴组织HLA-DR抗原的表达情况;另用流式细胞术检测研究组和对照组血清中CD3+、CD4+、CD8+及CD4+/CD8+的水平,并进行比较。结果①研究组外阴病变组织中HLA-DR抗原阳性表达率为30%,显著高于对照组正常外阴组织中HLA-DR抗原表达率(4.77%),差异有统计学意义(P<0.05)。②研究组血清中CD3+、CD4+的水平略低于对照组,CD8+的水平略高于对照组,差异均无统计学意义(P>0.05)。③研究组血清CD4+/CD8+的比值1.23±0.26,显著低于对照组CD4+/CD8+的比值(1.55±0.62),差异有统计学意义(P<0.05)。结论以HLA-DR为代表的遗传免疫因素可能与本病患者免疫功能紊乱,导致免疫应答过度,造成机体自身组织的损伤有关。
Objective To study the vulvar lichen sclerosus patients about the HLA - DR antigen expression in vulvar lesion tissue and T lymphocyte subpopulation levels in the peripheral blood and their functions in the vulvar lichen sclerosus pathogenesis. Methods From Dec 2006 to March 2008, Immunohistochemistry study of HLA - DR antigen in biopsy specimen of vulva was performed on 30 cases of VLS patients（ study group） and 21 cases of ob- stetrics and gynecology other benign lesions patients（ contrastive group ）. With Another Flow CytoMetry we detec- ted CD3＋ ,CD4＋ ,CD8＋ and CD4＋/CD8＋ levels in the study group and contrastive group,and the results were compared, Results （1）Study group vulvar lesion specimen, HLA - DR antigen positive level （ 30% ）is very higher than normal vular specimen HLA -DR antigen positive level（4.77% ） ,P 〈 0.05 ;The difference has statistical sig- nification. （2）CD3 ＋ , CD4 ＋ levels of the study group are slightly lower than the contrastive group ; CD8 ＋ level of the study group is slightly higher than the contrastive group, P 〉 0：05 ;The difference has no statistical signification. （3） CD4 ＋/CD8 ＋ level of the study group is 1.23 ± 0.26, significantly lower than CD4＋/CD8 ＋ level （ 1.55 ± 0.62） of the contrastive group, P 〈 0.05 ; The difference is significant statistically. Conclusion We can make a conclusion HLA - DR as a representative of the genetic and immune factors may be related to the incidence of vulvar lichen sclerosus ; At the same time, the incidence of this disease may be related to immune dysfunction in patients with ex- cessive immune response,resulting in the body＇s own injury tissues.
出处
《哈尔滨医药》
2010年第1期1-2,4,共3页
Harbin Medical Journal
基金
哈尔滨市科技局基金项目资助(2004AASCS196-29)
关键词
外阴硬化性苔藓
HLA-DR抗原
T细胞亚群
免疫组化
流式细胞术
vulvar lichen sclerosus
human leukocyte antigen - DRantigen
T iymphoeytes subpopulation
im- munohistochemistry
flow CytoMetry
