摘要
目的:探讨降钙素基因相关肽(CGRP)对脂多糖(LPS)诱导的小鼠巨噬细胞表达髓样细胞触发受体-1(TREM-1)的影响及其信号转导途径。方法:采用反转录-聚合酶链式反应(RT-PCR)观察巨噬细胞TREM-1mRNA表达量的变化,应用流式细胞术检测巨噬细胞膜表面TREM-1蛋白的表达。结果:CGRP对未受刺激的巨噬细胞表达TREM-1无明显影响,LPS可诱导巨噬细胞表达TREM-1;CGRP预处理呈剂量和时间依赖性上调LPS所致的巨噬细胞TREM-1mRNA的表达;同时,CGRP上调LPS所致的巨噬细胞膜表面TREM-1蛋白的表达;上述作用均可被PKC阻断剂H-7和PKA阻断剂H-89部分逆转(P<0.05)。结论:CGRP上调LPS诱导的巨噬细胞表达TREM-1,其胞内信号转导途径与PKA和PKC有关。
Objective To determine the effect of calcitonin gene-related peptide （CGRP） on triggering receptor expressed on myeloid cells-1 （ TREM-1 ） in the lipopolysaccharide （LPS） -induced macro- phages and its signal transduction pathway. Methods The levels of TREM-1 mRNA in the macrophages were observed by reverse transeription-polymerase chain reaction （RT-PCR）, and flow cytometry was performed to detect TREM-1 protein expression levels in the macrophages. Results CGRP had no regulating effect on the expression of TREM-1 in the macrophages ; LPS could up-regulate macrophages to express TREM-1; CGRP increased TREM-1 mRNA expression in LPS-induced maerophages in dose and time-dependent manner; CGRP increased TREM-1 protein expression in LPS-induced macrophages, which could be partially reversed by H-7 or H-89 （P 〈 0.05 ）. Conclusion CGRP can regulate the LPS-induced macrophages synthesis and secretion of TREM-1, and the intracellular signal transduction pathway is related to PKA and PKC.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2010年第2期100-106,共7页
Journal of Central South University ：Medical Science
基金
supported by the National Undergraduate Innovative Test Program(081053342)
关键词
降钙素基因相关肽
髓样细胞触发受体-1
脂多糖
巨噬细胞
calcitonin gene-related pcptide
triggering receptor expressed on myeloid cells-1
lipopolysaccharide
macrophage
