摘要
烤烟(Nicotiana tabacum L)嫩茎3~15层表皮和亚表皮薄细胞层在附加有适当激素配比的MS培养基上可一次培养成苗。薄细胞层一次培养成苗的适宜培养基为:MS+NAA0.1~0.3 mg/l +IBA0.1 mg/l +6-BA1.5~2.5 mg/l+3%蔗糖;BA浓度控制在2.5mg/l以下有利于培育壮苗。适宜的生根培养基为:1/2MS+2%蔗糖+NAA0.1~0.2 mg/l。
The epidermal and sub- epidermal thin cell-layer of flue-cured tobacco (Nicotiana tabacum L) tender stem in additional appropriate hormone combinations of the MS culture medium can take one-step shoot formation. MS + NAA 0.1-0.3 mg/L + IBA 0.1 mg/L + 6-BA 1.5-2.5 mg/L+ 3% Sucrose culture medium is suitable for one-step shoot formation in thin cell layer cultures. A BA concentration below 2.5mg/L is conducive to nurturing strong seedling. 1/2MS+2% Sucrose +NAA 0.1-0.2 mg/L culture medium are suitable for root induction.
出处
《中国农学通报》
CSCD
北大核心
2010年第6期51-53,共3页
Chinese Agricultural Science Bulletin
基金
湖南中烟工业有限责任公司科技委项目"利用花粉管通道导入野生烟草DNA创造烟草新种质"(2008-17)资助
关键词
烟草
薄细胞层
器官发生
tobacco
hin cell layer
organogenesis