摘要
目的用实时荧光定量PCR法检测低危型人乳头状瘤病毒HPV(如6/11型)和高危型人乳头状瘤病毒HPV(如16/18型),探讨其在宫颈癌防治方面的意义。方法采用FQ-PCR方法检测624例不同年龄组患者感染HPV6/11、HPV16/18型情况。结果624例标本中HPV6/11、HPV16/18检测的阳性率分别为26.3%,8.3%,低危型远高于高危型(P<0.01)。HPV6/11、HPV16/18阳性患者的年龄分布不同,年龄段的感染情况也不同,HPV6/11型感染主要集中在20～40岁,HPV16/18型感染者年龄均在41～50岁。结论荧光定量PCR检测低危型HPV和高危型HPV,方法简便准确,快速。
Objective To detect low-risk human papillomavirus（HPV） types （6/11） and high-risk HPV types（ 16/18 ） by the method of real-time fluorescence quantitation polymerase chain reaction（FQ-PCR） and investigate their roles in the prevention and cure uterine cervix cancer. Methods 624 patients from different age groups were tested their HPV types （6/11, 16/18） by the method of FQ-PCR. Results Of 624 women studied, the overall prevalence of low-risk and high-risk HPV DNA detection rate were 26.3% and 8.3% respectively. The positive rate of low-risk HPV types was significantly higer than that of high-risk HPV types （P〈0.01）. Moreover, the infection rate in different age groups were different. The women with HPV types 6/11 were distributed between the age 20-40, while the distribution of HPV types 16/18 positive women were clusted between the age 41 and 50 years old. Conclusion FQ-PCR is not only a simple and exact but also a prompt method to detect low-risk and high-risk HPV types.
出处
《分子诊断与治疗杂志》
2010年第1期35-36,共2页
Journal of Molecular Diagnostics and Therapy
关键词
人乳头瘤病毒
荧光定量PCR
基因分型
Human papilloma virus
Fluorescent quantitation polymerase chain reaction
Genotyping
