摘要
目的评价细胞间黏附分子．1单克隆抗体（1A29）对全脑缺血再灌注损伤大鼠的脑保护作用。方法健康成年雄性sD大鼠40只，体重180—200g，2—3月龄，采用四血管法制备全脑缺血再灌注模型。随机分为4组（n=10）：Ⅰ组于缺血前即刻股静脉注射同型对照抗体1rag／kg；Ⅱ组～Ⅳ组分别于缺血前即刻、再灌注即刻及再灌注4h时股静脉注射1A291mg／kg。于再灌注24h时进行神经功能损伤评分、脑组织多形核白细胞及单核细胞计数，并计算脑梗死面积百分比及脑组织含水量。结果与Ⅰ组比较，其余3组脑组织多形核白细胞计数、单核细胞计数、脑梗死面积百分比、含水量及神经功能损伤评分均明显降低（P〈0．01），3组间差异无统计学意义（P〉0．05）。结论缺血前或再灌注4h内静脉注射1A29均可减轻大鼠全脑缺血再灌注损伤。
Objective To investigate the cerebral protective effect of anti-intercellular adhesion molecule-1 （ anti-ICAM-1 ） antibody （ 1A29 ） in rats with global cerebral ischemia/reperfusion （I/R） injury. Methods Forty healthy SD rats weighing 180-200 g were randomly divided into 4 groups （ n = 10 each） ： group I received isotype-matched control antibody 1 mg/kg iv （control group）; group II , III, IV received 1A29 1 mg/kg iv before cerebral ischemia （group II ）, at the onset of reperfusion （ group m ） and at 4 h of reperfusion （ group IV） respectively. The global cerebral I/R was produced by 30 rain four-vessel occlusion followed by 24 h reperfusion. All animals were killed at 24 h of reperfusion. Their brains were removed for microscopic examination and measurement of W/D weight ratio, infarct size and neurological deficits （0 = no deficit,4 = unable to crawl with mental depression）. Results The PMN leukocyte and monocyte infiltration, water content of the brain and infarct size were significantly decreased in group II , III and IV as compared with control group. The neurological deficit scores were significantly lower in greup II , III, IV （1.6-1.8） than in control group （2.5）. Conclusion 1A29 antibody administered either before ischemia or during the 4 h of reperfusion can protect the brain against global cerebral I/R injury.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第1期83-85,共3页
Chinese Journal of Anesthesiology
基金
南京军区2007年度医学科技创新科研项目（07M025）
关键词
胞间黏附分子1
抗体
单克隆
脑缺血
再灌注损伤
Intercellular adhesion molecule-1
Antibodies, monoclonal
Brain ischemia
Reperfusion injury
