摘要
本研究旨在建立检测副猪嗜血杆菌(Hps)的新方法。根据GenBank上发表的不同血清型Hps的16S rRNA序列,找出高度保守区域,再针对该区域设计了4条特异性引物,建立了Hps环介导等温扩增法(LAMP)。试验结果表明,LAMP方法在恒温(63℃)条件下特异性强,比PCR方法敏感100倍,鉴于目前副猪嗜血杆菌分离培养和鉴定的复杂性,LAMP操作简单、成本低,整个扩增反应在1 h内即可完成,为检测Hps提供了一个快速简便的分子生物学方法。
Based on a series of 16S rRNA sequences of Haemophilus parasuis（Hps） published in GenBank,a conservative region was found.Four specific primers targeting the 6 distinct sequences of the conservative region were designed.A loop-mediated isothermal amplification（LAMP） for the rapid detection of Hps was established by using those specific primers.The results show that LAMP has a high specificity,and is more sensitive than PCR method under 63 ℃.Compared with the complex isolation and identification methods of Hps,LAMP is simple,low cost,and could be completed in 1 h.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第2期204-207,共4页
China Animal Husbandry & Veterinary Medicine
基金
广东温氏食品集团有限公司科研基金资助(C080921)
