摘要
采用RT-PCR方法对2006-2007年华南地区部分省份的疑似猪繁殖与呼吸综合征(PRRS)阳性病料进行克隆和测序,获得13个Nsp2基因的部分序列片段和14个ORF5基因片段。序列分析结果表明,所获得的13个Nsp2基因序列中均有2个区域存在明显缺失,核苷酸的同源性为96.9%～99.1%,与中国代表毒株CH-1a、美洲株代表毒株VR-2332的核苷酸序列同源性分别为85.1%～86.4%和67.2%～68.9%,与疫苗株MLV的同源性在67.6%～68.9%之间,而与欧洲株代表株LV的同源性在54.1%～55.9%之间;获得的14个ORF5基因序列不存在缺失现象,核苷酸的相似性为同源性为98.8%～99.7%,推导编码氨基酸同源性为96.5%～99%。与国内毒株相比,核苷酸同源性为85.2%～100%,氨基酸同源性为84.6%～99%;与中国代表毒株CH-1a、美洲型代表毒株VR-2332的核苷酸序列同源性分别在94.4%～95.2%和86.7%～87.9%之间,与疫苗株MLV的同源性在86.4%～87.6%之间,而与欧洲型代表株LV的同源性在54.1%～55.7%之间,推导编码的氨基酸序列同源性则分别在91.5%～93.5%,86.6%～88.6%,85.6%～87.6%,54.7%～56.2%之间。结果表明所获得序列的流行毒株均属于美洲型,进一步分析发现这些毒株可能均起源于同一个毒株,通过猪运输流通等途径在全国范围流行扩散。
Through RT-PCR amplification of tissue or blood sample from some of pig form of Huanan area,14 sequences of the ORF5 gene and 13 sequences of the partail Nsp2 gene were obtained.Sequencing and comparative analysis showed that two sequences exhibited deletion in the Nsp2 gene.There was 96.9% to 99.1% identity between those sequences of Nsp2 gene,sharing 85.1% to 86.4%,67.2% to 68.9%,54.1% to 55.9%,67.6% to 68.9% identity with CH-1a,VR-2332 LV and MLV respectively.The identity among those sequences of ORF5 gene was 98.8% to 99.7%,sharing 94.4% to 95.2%,86.7% to 87.9%,54.1% to 55.7%,86.4% to 87.6% identity with CH-1a,VR-2332,LV and MLV respectively.No deletion was exhibited in the 14 ORF5 gene sequences,and the sequences of ORF5 genes were analysed and compared with those of ATCC VR-2332,CH-1a,LV and MLV strains,there was 98.8% to 99.7% identity among those sequences of ORF5 gene,sharing 94.4% to 95.2%,86.7% to 87.9%,86.4% to 87.6% and 54.1% to 55.7% identity with CH-1a,ATCC VR-2332,MLV and LV respectively.And the homology comparision between the deduced amino acid sequence and other strains were 91.5% to 93.5%,86.6% to 88.6%,85.6% to 87.6%,54.7% to 56.2% respectively.The results showed that the prevalent PRRSV strains in China belonged to North American in genotype,and the result showed that those virus probable origin of one strain,and prevail throught the transportation of the pigs.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第2期96-100,共5页
China Animal Husbandry & Veterinary Medicine
基金
2007年度广东省科技计划项目(2007A020300006)
