摘要
目的探讨EB病毒相关抗体和DNA的联合检测在类风湿关节炎（RA）诊断中的应用。方法收集102例RA患者和206例健康人群的血清。运用酶联免疫吸附法（ELISA）检测血清中EB相关抗体VCA—XgG和EA—IgG，同时用实时定量PCR法（FQ—PCR）检测血清中游离的EBV—DNA含量，并对结果进行综合分析。结果单项指标在RA诊断中的灵敏度、特异度、准确率、阳性预测值和阴性预测值分别为：VCA—IgG91．2％，87．9％，89．0%，95．3％，78．8％；EA—IgG70．6％，90．3％，83．8%，86．1％，78．3％；EBV—DNA44．1％，93．2％，76．9％，77．1％，76．2％；三项指标联合检测则分别为99．0％，86．9％，90．9％，78．9％和99．4％。结论单项指标中VCA—IgG最佳，二种EB病毒相关抗体和血清游离EBV—DNA的联合测定可弥补各单项指标的缺点，可更好地作为RA的辅助诊断指标。
Objective To study the diagostic value of combined determination of Epstein-Barr Virus-related antibodies and EBV-DNA in Rheumatoid Arthritis（RA）. Methods The sera of 102 patients with RA and 206 control donors were collected. The sera antibodys level against EBV-related （VCA-IgG and EA-IgG） were detected by using solid phase enzymelinked immunosorbent assay（ELISA）,and then the EBV-DNA in sera was detected by using fluorescence quantitative polymerase chain reaetion（FQ-PCR）. Results When evaluated individually,the diagnostic indexes （sensitivity,specificity, accuracy, predictive value of positive, and predictive value of negative） were VCA-IgG 91.2 %, 87.9 %, 89.0 %, 95.3 %, 78.8% ;EA-IgG 70. 6% ,90.3% ,83.8% ,86.1% ,78.3% ;EBV-DNA 44. 1% ,93.2% ,76.9% ,77.1% ,76.2%. Combine detection of there indexes were 99.0%,86.9%, 90. 9%, 78. 9% and 99.4% respectively in RA. Conclusion VCA-IgG was the best in the three indexes. Combine detection of two Epstein-Burr Virus-related antibodies （VCA-IgG and EA-IgG） and EBV-DNA in sera were better than single index in some patients with RA.
出处
《现代检验医学杂志》
CAS
2010年第1期136-137,140,共3页
Journal of Modern Laboratory Medicine
基金
佛山市卫生局课题（编号2008089）.
关键词
EB病毒
类风湿关节炎
相关性
epstein-barr virus
rheumatoid arthritis
diagnosis
