摘要
目的了解人肝细胞癌中细胞凋亡的形态与分布规律,探索细胞凋亡标记率和增殖标记率与肝细胞癌组织学分级的关系。方法对21例人肝细胞癌和17例慢性肝病分别作TUNEL 标记和 PCNA 免疫组化检测,采用图像细胞仪作阳性细胞计数。结果 (1)细胞核染色质浓缩、边聚、胞浆嗜酸性变及核周空晕与 TUNEL 标记有极好的相关性;(2)凋亡细胞多呈弥散分布,以癌坏死区周围多见,包膜浸润处少见;(3)增殖细胞率和凋亡细胞率与肝癌组织学分级呈正相关,Ⅲ级组肝细胞癌中增殖细胞率为27.8±6.4,高于Ⅱ级组的14.1±3.5(P<0.01)和Ⅰ级组的7.4±0.8(P<0.001);Ⅲ级组肝细胞癌中凋亡细胞率为2.1±0.97,高于Ⅱ级组的0.92±0.26(P<0.05)和Ⅰ级组的0.45±0.08(P<0.001);(4)包膜浸润和门脉癌栓组凋亡细胞率和增殖细胞率均高于无包膜浸润和门脉癌栓组。结论本研究提示细胞凋亡和细胞增殖与肝细胞癌组织学分级、包膜浸润和门脉癌栓密切相关。
Objective To investigate the morphology and distribution of the apoptotic cells,and the relationship between apoptosis,proliferation and the histologic grading of human hepatocellular carcinoma(HCC).Method 21 cases of human HCC and 17 cases of chronic liver disease were analyzed by labeling the apoptotic cells with TUNEL technique and PCNA by immunohistochemical method.Results(1)There was a positive correlation between the TUNEL labeling index(TUNEL LI)and nuclear condensation,concentrated chromatin and eosinophi- lic cytoplasm;(2)Most of the apoptotic cells were scattered in distribution or located around the necrotic tumor area,but being less in tumor infiltrating area;(3)The TUNEL LI of grade Ⅲ,Ⅱ and Ⅰ of HCC were 2.1±0.97,0.92±0.26 and 0.45±0.08,and the PCNAL LI were 27.8±6.4,14.1±3.5,7.42±0.8,respectively.Both TUNEL LI and PCNAL LI were positively correlated with histologic grading of the HCC,P<0.05～ 0.001);(4)The TUNEL LI and PCNA LI of the capsule infiltrated group and portal vein tumor thrombus group were higher than those in the noninfiltrated and absence of tumor thrombus groups.Conclusion This study shows that the apoptotic LI and PCNA LI are highly positive and correlated with the histologic grading,capsuler infiltration and portal vein tumor thrombus.Apoptosis may thus play an important role in the growth pattern of human HCC.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1998年第6期334-336,共3页
Chinese Journal of Digestion
关键词
癌
肝细胞
细胞凋亡
增殖细胞核抗原
Carcinoma,hepatocellular
Proliferating cell nuclear antigen
Cell apoptosis
