摘要
为建立复方穿心莲片中穿心莲内酯和脱水穿心莲内酯的含量测定方法，采用高效液相色谱法。使用ODS（250mm×4．6mm，5μ没）分析柱，流动相为甲醇-水（62：38），流速为1mL／min，检测波长为225nm，柱温30℃。结果表明：穿心莲内酯和脱水穿心莲内酯的线性范围为0．1—1．0μg。制剂中穿心莲内酯和脱水穿心莲内酯的含量限度规定不得少于2．0mg／片。该方法简便可行，快速准确，重现性好，可很好的应用于复方穿心莲片中穿心莲内酯和脱水穿心莲内酯的含量测定。
To establish a reversed-phase HPLC method for the quantitative determination of andrographolide and dehydroandrographolide in Compound Chuanxinlian Tablets, the experimental conditions were as follows： PhenomenexODS column （250 mm × 4.6 mm,5 μm）, mobile phase： methanol-water（ 62：38 ）, flow rate ： 1 mL/min, detective wavelength ： 225 nm, column temperature：30 ℃. The calibration curve of andrographolide and dehydroandrographolide CRS showed a good linearity. The test solution was stable in methanol within 24 h. The recovery rates were found to be for andrographol- ide and dehydroandrographolide CRS were 98.3% and 97.8% ,and RSD for them were 1.86% and 2.15% ,respectively. The established method is simple feasible, fast, accurate, reproducible and reliable to operate and suitable for the quality control of Compound Chuanxinlian Tablets.
出处
《天然产物研究与开发》
CAS
CSCD
2009年第B10期431-433,共3页
Natural Product Research and Development
