摘要
目的比较肺炎支原体(MP)抗体检测法与荧光定量检测方法(PCR)在肺炎支原体感染中的应用。方法对本院住院部362例下呼吸道感染的患儿的血液标本运用酶联免疫吸附试验(ELISA)法进行抗体检测(MP-IgM),同时运用PCR方法对其痰液标本进行检测(FQ-MP-DNA)。结果血MP-IgM与痰FQ-MP-DNA两种方法差异无统计学意义(P>0.1),但后者对1岁以下的患者检出率更高(P<0.005)。结论两种检测方法结合可以取长补短,提高支原体感染的检出率。
Objective To comparion of the method between mycoplasma pneumoniae-specifie IgM（MP-IgM） antibody detection test and fluorescent quantitation PCR （PCR） test in the treatment of infection of Mycoplasma pneumoniae. Methods The method of ELISA was employed to detect MP-IgM antibody in the blood of 362 cases of respiratory infection, at the same time, the I）NA of sputum of those patients（FQ-MP DNA） was detected by means of PCR. Results MP IgM detection test and FQ MP DNA test have almost resemble positive rate of detection. The latter has higher positive rate in those children of 〈1 years older than the former. Conclusion To employ both methods can get higher positive rate of detection at the same time.
出处
《检验医学与临床》
CAS
2010年第5期412-413,共2页
Laboratory Medicine and Clinic
关键词
支原体感染
支原体抗体
荧光定量检测方法
infection of mycoplasma pneumoniae
mycoplasma pneumoniae specific IgM antibody
PCR
