摘要
将变链菌NG_8ScrA基因432bp扩增片段纯化后,采用随机引物法标记成DNA探针,与13株变链菌相应的扩增产物进行Southern杂交,结果均出现杂交信号。进一步采用不对称PCR法测序,结果与文献报道的序列一致。表明PCR扩增产物特异性好,ScrA基因在变链菌及部分口腔常居菌间存在广泛同源性。
The 432bp amplified DNA fragments of streptococcus mutans NG8 strain was purified and with which to prepare labeled DNA probe by a random priming procedure. The conclusion indicated the probes strongly hybridized with all the streptococcus mutans. Meanwhile, Generation of single- stranded DNA by PCR and its application to direct sequencing of the amplified fragments. The conclusion demonstrated similar as described previously by Sato, et al( 1989). The ScrA gene was existed in streptococcus mutans and some oral permanent bacteria.
出处
《现代口腔医学杂志》
CAS
CSCD
1998年第4期251-253,共3页
Journal of Modern Stomatology
