摘要
为研究丙戊酸钠对脑组织不同区域γ－氨基丁酸（ＧＡＢＡ）生成的机制，分别给大鼠ｉｐ２００ｍｇ／ｋｇ丙戊酸钠、２００ｍｇ／ｋｇ丙戊酸钠＋１００ｍｇ／ｋｇ氨基羟乙酸（ＡＯＡＡ），３０ｍｉｎ后断头处死，观察下丘脑、小脑、延髓、大脑皮层四个脑区谷氨酸脱羧酶（ＧＡＤ）、γ－氨基丁酸转氨酶（ＧＡＢＡ－Ｔ）活性及ＧＡＢＡ含量的变化。结果表明，丙戊酸钠既抑制ＧＡＢＡ－Ｔ也能激活ＧＡＤ，显著提高下丘脑、大脑皮层两个脑区的ＧＡＢＡ含量。虽然四个脑区的ＧＡＢＡ－Ｔ活性得到显著的抑制，但ＧＡＢＡ并没有相应地显著提高；仅在ＧＡＤ活性显著升高的下丘脑及大脑皮层两个脑区的ＧＡＢＡ含量显著增加。ＡＯＡＡ加丙戊酸钠组与单独ＡＯＡＡ组比较，各脑区ＧＡＢＡ－Ｔ活性无明显差别变化，仅在下丘脑中的ＧＡＤ活性显著提高，相应的ＧＡＢＡ含量也显著增加。提示丙戊酸钠主要是通过激活ＧＡＤ，从而提高脑组织ＧＡＢＡ的含量。
The effects of the sodium valproate on the γ aminobutyric acid (GABA) metabolism in 4 brain regions in rat were studied by administering the sodium valproate at a dose of 200mg/kg and 100mg/kg aminooxyacetic acid( AOAA) with 200mg/kg sodium valproate Sodium valproate could suppress the activity of GABA T in a 4 brain regions and significanly enhanced the activity of GAD and increased the GABA in the hypothalamus and cerebral cortex in rats Sodium valproate at 30 min prior to injection of AOAA (complete inhibition of GABA degration) shows the activity increased of GAD and elevating GABA level significantly in the hypothalamus These data suggest that the sodium valproate increasing GABA in this region may be induced by rather activiting GAD than suppressing GABA T
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1998年第5期380-382,共3页
Journal of China Pharmaceutical University
关键词
氨基丁酶
谷氨酸脱羧酶
GABA-T
丙戊酸钠
γ Aminobutyric acid
L Glutamate decarboxylase
Aminotransferase
Sodium valproate
