摘要
通过差别筛选HgCl2胁迫下的菜豆叶片cDNA库，分离出7组不同的cDNA克隆（Phaseolusvulgarisstress-relatedprotein，PvSR1～7）。cDNA序列和同源性分析结果表明：PvSR1编码富含脯氨酸细胞壁蛋白（PRP），PvSR2和PvSR7编码新的HgCl2胁迫相关蛋白，PvSR3编码脱水蛋白（dehydrin），PvSR4编码病原相关（PR）蛋白，PvSB5编码polyubiqui－tin，PvSR6编码DuaJ－like蛋白。HgCl2胁迫可强烈地请导PvSR2和PR蛋白基因的表达，并能提高PRP，、dehydrinlike和polyubiq－uitin基因的转录水平。这些蛋白质共同作用可能对维持细胞的正常代谢和抵抗重金属胁迫方面有重要作用。
Seven different cDNA framilies were isolated from a mereuric-chloride-treated bean (Phaseolus vulgaris L. )cDNA library by differential screening using cDNAs derived from treated and untreated plants. Transcriptionally activated cDNA clones are designated PvSR (Phaseolus vulgaris stress-related protein). The sequences of the cDNA inserts and homological analysis showed that they encode proline-rich protein (PvSR1 ), dehydrin (PvSR3 ), patho.,genesis-related protein (PvSR4), polyubiquitin (PvSR5 ), DnaJ-like protein (PvSR6) and two new HgCl2-stress-re-lated proteins (PvSR2 and PvSR7 )(Table 1 ). Northem blotting analysis revealed that the gene expression of PvSR2 and pathogenesis-related protein were strongly induced in leaf tissue by HgCl2stress, the transcript levels of prolinerich protein, dehydrin, polyubiqUitin,DnaJ-like protein and PvSR7 were greatly enhanced too (Fig. 1 ) , The various gene products might function to protect cell from heavy-metal stress.
基金
青年863基金
留学回国人员启动经费资助
关键词
菜豆
重金属
DNA序列分析
胁迫
蛋白
kidney bean (Phaseolus vulgaris L.),heavy metal, DNA sequence and enalysis, stress -related protein
