摘要
目的探讨HIF-1α和Rac-1在胃癌组织中的表达及临床意义。方法应用免疫组织化学法检测63例人胃癌组织和40例癌旁组织标本中HIF-1α和Rac-1的表达,采用半定量计数法判定,并结合临床资料进行分析。结果HIF-1α和Rac-1在胃癌组织中表达的阳性率分别为68.25%和63.49%,而在癌旁组织中的阳性率分别为5.00%和17.50%(P<0.01);HIF-1α和Rac-1在胃癌组织中的表达与TNM分期、浸润深度和淋巴结转移有关(P<0.05),而与性别、年龄、原发部位及肿瘤大小无关(P>0.05),且Rac-1与胃癌组织分化程度呈正相关,而HIF-1α与胃癌组织分化程度无显著相关性;在胃癌组织中HIF-1α和Rac-1的表达呈正相关。结论胃癌组织中HIF-a和Rac-1的表达显著增加,与胃癌的侵袭性生物学行为密切相关,两者的联合检测可能成为预测胃癌转移及预后的重要指标。
Objective To explore the expression and clinical significance of hypoxia-inducible factor-1α （HIF-1α）and Rasrelated C3 botulinum toxin substrate 1 （Rac-1） protein in gastric cancer and normal gastric tissues. Methods Immunohistochemical method was used to detect the expression of HIF-1α and Rac-1 protein in 63 specimens of gastric cancer, 40 specimens of normal gastric tissues. Results The positive rates of the expression of HIF-1α and Rac-1 in gastric cancer tissue were 68. 25% and 63.49% respectively. The expressions of them in normal tissue were 5.00% and 17. 50% respectively. HIF-loL and Rac-1 in gastric cancer tissues were significantly higher than those in adjacent noncancer tissues（P 〈0. 01 ）. The expression of HIF-1α and Rac-1 were related with stage of TNM, invasion degree,lymph node metastasis （P 〈 0. 05 ）, but there was no obvious correlation with its expression and the sex, age of patient, primary pathological location or size of gastric cancer（ P 〉 0. 05 ）. The expression of HIF-1α was also related to differentiated degree of histologic type（P 〈 0. 05 ） , while Rac-1 was not. The expression of HIF-1α was positively correlated to that of Rac-1 in gastric cancer. Conclusion HIF-1α and Rac-1 are found to be highly expressed in the tissues of the gastric cancer, which are correlated with the biological behavior of gastric cancer, a joint detection of which might be an important marker and prognostic factor in gastric cancer.
出处
《临床肿瘤学杂志》
CAS
2010年第2期109-113,共5页
Chinese Clinical Oncology
关键词
胃癌
HIF-1Α
Rac-1
免疫组织化学
Gastric cancer
Hypoxia-inducible factor-1α （ HIF-1α）
Ras-related C3 botulinum toxin substrate 1 （ Rac-1 ）
Immunohistochemistry
