摘要
目的探讨芪莲汤颗粒剂(QiLian soup-granula)对糖尿病肾病(diabetic dephropathy,DN)大鼠肾脏病变的保护作用及机制。方法采用高脂饲料联合链脲佐菌素(streptozotocin,STZ45 mg/kg)腹腔注射法建立DN大鼠模型,模型成功后分组给药。设正常对照组、模型对照组、消渴丸组、芪莲汤颗粒剂高剂量组、芪莲汤颗粒剂低剂量组。按试验设计剂量灌胃给药,1次/d,每周6d。连续给药4个月后检测空腹血糖、血肌酐、做糖耐量试验、尿素氮、尿肌酐、尿蛋白。取肾脏组织作病理、免疫组织化学法检测肾小球Ⅳ型胶原蛋白的表达。结果芪莲汤颗粒剂可以显著降低大鼠空腹血糖、尿素氮、血肌酐和尿蛋白。并能抑制DN大鼠肾组织Ⅳ型胶原蛋白表达。结论芪莲汤颗粒剂对DN大鼠具有很好的治疗作用,可以延缓DN病理进程。该作用可能与降血糖及降低胰岛素抵抗和抑制肾组织Ⅳ型胶原表达有关。
Objective To research the protection of QiLian soup-granula（QLS） on rats with diabetic nephropathy （DN） and the underlying mechanism.Methods Seventy SD rats were randomly divided into 2 groups： 60 rats treated with high calorie feeds and streptozotocin （STZ） to establish the DN model,and 10 rats in normal group.DN rats were randomly divided into model control group,XKW group （xkw,0.80g/（kg·d））,QLS-H group （QLS,10g/（kg·d）） and QLS-L group （QLS,5g/（kg·d））.DN rats underwent drug treatment for 4 months.Then the fasting blood glucose （FBG）,urea nitrogen （BUN）,urine protein （Upro） and urine carnine （UCr） were tested,the oral glucose tolerance test （OGGT） was performed,and the expression of Ⅳ-C on nephridial tissue was measured by immunohistochemistry.Results QiLian soup-granula can significantly decrease the FBG,BUN,UCr,Scr and Upro and increased the sugar tolerance.QiLian soup-granula can decrease the expression of Ⅳ-C in nephridial tissue.Conclusion QiLian soup-granula had protection on DN-rats and can delay the proceeding of DN.This protection may be related to the decrease of FBG,BUN,UCr,Upro,the expression of Ⅳ-C in nephridial tissue and the increase of sugar tolerance.
出处
《华南国防医学杂志》
CAS
2010年第1期37-41,共5页
Military Medical Journal of South China
关键词
芪莲汤颗粒剂
糖尿病肾病
Ⅳ型胶原
QiLian soup-granula
Diabetic nephropathy
Type Ⅳ collagen
