摘要
目的:评价自体与新鲜异体骨软骨镶嵌移植两种方法修复全层关节软骨缺损的生物学特性和修复效果。方法:采用完全随机设计,将16只新西兰大白兔的左右后肢制成全层软骨缺损模型,分别进行自体骨软骨镶嵌移植、同种异体骨软骨镶嵌移植修复。术后第12周处死动物取材,分别进行膝关节活动度测定、大体观察、光镜观察及Wakitani组织学评分法,数据行统计学分析。结果:膝关节伸屈活动度、大体观察、组织学光镜及组织修复评分显示自体骨软骨移植在第12周时能以类透明软骨组织修复缺损,而新鲜同种异体骨软骨移植为纤维肉芽组织。结论:自体骨软骨镶嵌移植可以修复兔关节软骨的缺损。新鲜无处理的同种异体骨软骨镶嵌移植修复关节软骨缺损不可行,其排斥反应及吸收破坏严重。
Objective： To compare and evaluate the differences of potentialities and biological characteristics between autologous osteochondral grafts and allografts in the defects of articular cartilage in weight - bearing joints, for the purpose of proposing the experimental basis for its clinical application. Methods：Sixteen adult rabbits were randomly divided into 2 groups. In each group, autologous cartilage transplant and allotransplant were applied for repairing size - matched, full - thickness articular cartilage defects on the femoral condyle of the knees, respectively. The reconstructed tissues were observed by gross and optical microscope observation and were analyzed morphologically at 12 weeks after operation. Results：The autologous osteochondral grafts repaired the lesions with hyaline -like cartilage at 12 weeks ,while those with the allografts were filled with fibrous tissues. Conclusion： Autologous osteochondral grafts can successfully repair the articular cartilage defects in rabbits. Allografts without premedication are not an alternative because of its severe rejection and absorption.
出处
《浙江中西医结合杂志》
2010年第3期138-140,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
杭州市科技发展计划重点专科专病专项(No.20070433Q20)
