摘要
目的:研究云南红药对类风湿性关节炎模型大鼠足肿胀的抑制作用以及对大鼠血清IL-1β和TNF-α含量的影响,并探讨其作用机制。方法:将60只SD大鼠随机分成空白组、模型组、阳性对照组以及云南红药高、中、低剂量组。除空白组外,用Ⅱ型胶原蛋白诱导其余各组大鼠建立类风湿性关节炎模型。阳性对照组灌胃给予地塞米松磷酸钠2 mg.kg-1,云南红药高、中、低剂量组分别灌胃给予云南红药0.4、0.2和0.1 g.kg-1,空白组、模型组灌胃给予生理盐水0.01 mL.g-1,所有大鼠均为每日给药1次,连续28天。记录各组大鼠右后足肿胀程度变化情况,并于第28天处死大鼠,检测其血清IL-1β和TNF-α的含量。结果:模型组大鼠在注射Ⅱ型胶原蛋白后的第15天,云南红药高、中、低3个剂量组的大鼠分别在注射Ⅱ型胶原蛋白后的第22、20、18天右足肿胀率达100%;与模型组相比,云南红药高、中剂量在给药16天后即能显著抑制大鼠足肿胀(P<0.05),而云南红药低剂量组在给药24天后才产生显著抑制作用(P<0.05);云南红药高、中剂量组大鼠的血清IL-1β和TNF-α的含量明显低于模型组(P<0.01),模型组大鼠血清中IL-1β和TNF-α的含量分别为152.34 ng.L-1和31.76μg.L-1,高剂量组分别为69.64 ng.L-1和15.37μg.L-1,中剂量组分别为82.21 ng.L-1和19.44μg.L-1。结论:云南红药能够有效阻止类风湿性关节炎的发生、发展,其作用机制可能与下调致炎因子IL-1β和TNF-α的水平有关。
Objective：To study the effects of Yunnan-hongyao（HY） on the paw edema of rheumatoid arthritis rats model,as well as the levels of their serum IL-1β and TNF-α.Methods：60 SD rats were randomly divided into 6 groups,including blank group,model group,positive control group,different HY groups at low,middle and high dose.The model of type II collagen（C II）-induced arthritic（CIA） rats was established in each group except the blank group.The rats in positive control group were administered dexamethasone sodium phosphate（2 mg ·kg-1,qd,for 28 consecutive days） by intragastric perfusion,the rats in HY groups were given HY（0.4,0.2 and 0.1 g ·kg-1,qd,respectively,for 28 consecutive days） by intragastric perfusion,the rats in blank group and model group were given normal saline（0.01 mL ·g-1,qd,for 28 consecutive days） by intragastric perfusion.Then the inhibitory effects on paw edema were observed each day,and serum IL-1β and TNF-α level were determined after sacrificing the rats on 28th day.Results：On the 15th day after injecting CII,the swelling rate of right paw in model group reached 100%,while those were respectively on the 22th,20th,18th day in high,medium dose and low dose group.Compared with the model group,the paw edema was inhibited significantly in high and medium dose group on the 16th day after administration（P〈0.05）,while that was significantly inhibited on 24th day in low dose group（P〈0.05）.Compared with model group,the levels of serum IL-1β and TNF-α were obviously lower in high and medium dose group（P〈0.01）.The levels of serum IL-1β and TNF-α in model group were 152.34 ng ·L-1 and 31.76 μg ·L-1,respectively,while those were respectively 69.64 ng ·L-1 and 15.37 μg ·L-1 in high dose group,82.21 ng ·L-1 and 19.44 μg ·L-1 in medium dose group.Conclusion：Yunnan-hongyao can effectively treat rheumatoid arthritis,the mechanism of which may be related to lowering serum IL-1β and TNF-α levels.
出处
《药学进展》
CAS
2010年第2期81-84,共4页
Progress in Pharmaceutical Sciences
