摘要
目的探讨白藜芦醇(Res)对动脉粥样硬化兔心肌组织线粒体途径凋亡的影响。方法健康雄性新西兰白兔70只,随机分为5组:正常组、病理对照组、病理+低剂量Res组(4 mg.kg-1.d-1),病理+中剂量Res组(8 mg.kg-1.d-1)、病理+高剂量Res组(16 mg.kg-1.d-1)。分笼饲养12周后,取左心室尖部心肌组织。H-E染色观察心肌组织形态学改变,原位末端标记法检测心尖部细胞凋亡率,半定量RT-PCR检测bax、bcl-2的mRNA变化。结果H-E染色下各组细胞形态学改变不明显;病理对照组细胞凋亡指数高于正常组(P<0.01)和Res干预组(P<0.01),Res作用下凋亡指数下降呈剂量依赖性(P<0.05);病理对照组bax、bcl-2mRNA表达量较正常组升高(P<0.01),而加入Res干预后,bax的表达量明显下降,bcl-2表达升高(P<0.01)。结论Res可以减少促凋亡基因bax的表达,增加抗凋亡基因bcl-2的表达,降低Bax/bcl-2比值,抑制心肌细胞的凋亡。
Objective To investigate the effect of resveratrol on myocardial mitochondria-mediated apoptosis in rabbits with artherosclerosis. Methods Seventy male New Zealand white rabbits were randomly divided into 5 groups ：A for normal control group ,B for pathological control group, C for pathological control group treated with low dosage resveratrol （4 mg·kg^-1·d^-1） ,D for pathological group treated with mid dosage resveratrol （8 mg·kg^-1·d^-1） and E for pathological group treated with high dosage resveratrol （16 mg·kg^-1·d^-1 ）. At the end of the 12th week, left ventricular tissues were analyzed by histopathological examination, and apoptotic cell were detected by Terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling. The mRNA expression of bax and bcl-2 were detected by semiquantitative reverse transcriptase polymerase chain reaction. Results There were no obvious morphological changes in each group during H-E staining. Apoptotic index in group B was significantly increased when compared with group A, C, D and E （P〈0.01）. Resveratrol can inhibit apoptosis of myocytes in a dosedependent manner （P〈0.05）. The mRNA expression of bax and bcl-2 were significantly increased in group B than in group A （P〈0.01）. The mRNA expression of bax was significantly decreased after resveratrol intervention, simultaneously the mRNA expression of bcl-2 was increased （P 〈0.01 ）. Oonclusions Resveratrol can promote expression of the anti-apoptotic gene bcl-2 and decrease the pro-apoptotic gene bax in myocardial cells of rabbits with atherosclerosis. Resveratrol may inhibits myocardium apoptosis by reducing bax/bcl-2 ratio.
出处
《福建医科大学学报》
2009年第6期435-439,共5页
Journal of Fujian Medical University
基金
卫生部科学研究基金-福建省卫生教育联合攻关计划(WKJ2005-2-017)
福建省自然科学基金(Z0516069)
