摘要
目的:观察环维黄杨星D(Cyclovirobuxine D,CVB-D)一甲醇结晶体溶液与CVB-D原料溶液对豚鼠单个心室肌细胞动作电位(Action Potential,AP)的影响。方法:Langendorff灌流系统灌流离体心脏,急性酶解法获得单个心室肌细胞;全细胞膜片钳技术记录膜电位。结果:CVB-D一甲醇结晶体引起静息电位(RP)从(-80.8±6.6)mV减小至(-79.7±6.7)mV(n=6,P<0.05),动作电位0相除极幅度(APA)从(119.1±6.5)mV下降至(115.7±5.8)mV(n=6,P<0.01),复极至50%时动作电位时程(APD50)从(419.6±132.9)ms延长至(554.3±135.7)ms(n=6,P<0.01),复极至90%时动作电位时程(APD90)从(445.4±132.3)ms延长至(584.1±134.1)ms(n=6,P<0.01),复极至50%到复极至90%的动作电位时程(APD50-90)从(25.8±6.4)ms延长至(29.8±7.8)ms(n=6,P<0.05),3相复极斜率(V3)从(-1.9±0.5)V.s-1减慢至(-1.6±0.4)V.s-1(n=6,P<0.01)。CVB-D原料溶液组AP相关指标的变化趋势与CVB-D一甲醇结晶体溶液组一致。结论:CVB-D引起RP减小、APA下降、APD50延长、APD90延长、APD50-90延长和V3减慢,以上影响可能是CVB-D具有正性肌力作用和抗心律失常作用的药理机制。CVB-D一甲醇结晶体的药物效应更强。
Objective：To investigate the effect of menthanol crystal of cyclovirobuxine D（CVB-D） and raw material of CVB-D on action potentials in isolated guinea pig ventricular myocytes.Methods：The dissociated myocytes were enzymatically obtained from the guinea pig hearts and the action potentials were examined by whole-cell patch-clamp technique.Results：Menthanol crystal of CVB-D decreased resting potential（RP） from-80.8±6.6 mV to-79.7±6.7 mV（n=6,P0.05）,decreased action potential amptitude（APA） from 119.1±6.5 mV to 115.7± 5.8 mV（n=6,P0.01）,prolonged action potential duration of repolarization 50%（APD50） from 419.6±132.9 ms to 554.3±135.7 ms（n=6,P0.01）,prolonged action potential duration of repolarization 90%（APD90） from 445.4±132.3 ms to 584.1± 134.1 ms（n=6,P0.01）,prolonged action potential duration of repolarization 50% to repolarization 90%（APD50-90） from 25.8±6.4 ms to 29.8±7.8 ms（n=6,P0.05）,and decreased decay slope of phase 3（V3） from-1.9±0.5 V·s-1 to-1.6±0.4 V·s-1（n=6,P0.01）.Raw material of CVB-D resulted in same changes,all of which had their statistical significance.Conclusion：CVB-D decreased RP,APA and V3 ;and prolonged APD,which may be its mechanism of positive inotropic effect and antiarrhythmic effect.Menthanol crystal of CVB-D had more intense drug influence than raw material of CVB-D.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2009年第6期39-42,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
河南省教育厅自然科学基金资助项目(项目编号2009A360020)
