摘要
目的:研究延胡索乙素单体及延胡索提取物中延胡索乙素在大鼠肝微粒体中的酶促反应动力学,考察单体及提取物中延胡索乙素在大鼠肝微粒体代谢的差异。方法:采用高效液相法测定单体及延胡索提取物中延胡索乙素在体外代谢系统中代谢,计算酶动力学参数。结果:延胡索乙素单体在大鼠肝微粒体内代谢反应的最大反应速率Vmax、米氏常数Km和肝内消除率CLint分别为(4.19±0.87)μmol/L.(min.mg)、(172.68±19.24)μmol/L、(0.024±0.008)L.(min.mg);延胡索提取物中延胡索乙素在大鼠肝微粒体内代谢反应的最大速率Vmax、米氏常数Km和肝内消除率CLint分别为(0.12±0.02)μmol/L.(min.mg)、(5.44±1.74)μmol/L、(0.022±0.006)L.(min.mg)。结论:延胡索提取物中的延胡索乙素与延胡索乙素单体在大鼠肝微粒体中的代谢动力学有差别,这种差别可能是提取物中其它成份对代谢酶的作用引起的。
Objective：To study the enzyme kineics of corydalis extract and dl-tetrahydropalmatine metabolism in rat liver microsomes,to investigate the different of enzyme kineics of dl-tetrahydropalmatine metabolism in monomer and corydalis extract in rat liver microsomes.Method：A HPLC method was used to determinate dl-tetrahydropalmatine and the enzymatic reaction kinetics of dl-tetrahydropalmatine and corydalis extract in rat liver microsomes,and the enzyme kinetic parameters of dl-tetrahydropalmatine in monomer and corydalis extract was calculated.Results：The Vmax,Km,and CLint of the monomeric dl-tetrahydropalmatine were（4.19±0.87）μmol/L·（min·mg）,（172.68±19.24）μmol/L,（0.024±0.008）L·（min·mg）,respectively.The Vmax,Km,and CLint of the dl-tetrahydropalmatine in corydalis extract were（0.12±0.02）μmol/L·（min·mg）,（5.44±1.74）μmol/L,（0.022±0.006）L·（min·mg）.Conclusion：The enzyme kineics of dl-tetrahydropalmatine and corydalis extract in rat liver microsomes has difference.These differences may be caused by the interaction of medical ingredients or the effect of other components in corydalis extract on dl-tetrahydropalmatine.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2009年第6期30-33,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
江西省自然科学基金资助项目(2007GZY0932)
关键词
延胡索乙素
延胡索
代谢
肝微粒体
高效液相色谱
dl-tetrahydropalmatine
Corydalis extract
metabolism
liver microsomes
HPLC
