摘要
选用3只1岁半、平均体重24 kg,安装永久瘤胃瘘管的甘肃高山细毛羊羯羊,按3×3拉丁方试验设计,每个循环19 d(预试期10 d,正试期9 d),研究饲粮中红豆草缩合单宁水平对绵羊瘤胃代谢的影响。试验共设3个处理饲粮,其中红豆草缩合单宁水平依次是Ⅰ为0.00 g/kg DM、Ⅱ为1.52 g/kg DM、Ⅲ为3.03 g/kg DM。试验结果表明,3个处理的瘤胃液pH均值(依组次为6.24±0.32,6.13±0.28和6.13±0.37)无显著差异(P>0.05);处理Ⅱ、Ⅲ瘤胃液总挥发性脂肪酸(TVFA)呈高于Ⅰ的趋势(P=0.099);处理Ⅲ的乙酸摩尔比低于处理Ⅰ(P<0.05)与Ⅱ(P=0.074),其丙酸摩尔比较高(P=0.088),乙酸/丙酸较低(P=0.096);各处理间瘤胃液总氮、蛋白氮、细菌氮、氨态氮、尿素氮的平均浓度差异均不显著(P>0.05)。因此,本试验结果表明,含红豆草缩合单宁3.03 g/kg DM的饲粮对绵羊瘤胃纤维素分解微生物的活性产生一定程度的抑制,而分解淀粉的微生物活性被增强。
Three, one and a half years old, High Mountain Fine-wool wethers with an average weight of 24 kg, were fitted with permanent rumen fistulas. They received a random sequence of three treatments in a 3 × 3 Lat in square design with feeding cycles of 19 days （10 d preliminary period and 9 d sampling period） to study the effect of condensed tannin of Onobrychis viciaefolia hay on rumen metabolism. Condensed tannin （CT） contents in treatments I , III and III were 0.00, 1.52, and 3.03 g/kg of DM, respectively. Three similar sheep were used to measure in situ degradation of DM and CP of three diets. 1） The average rumen pH values of the three treatments were 6.24±0.32, 6.13±0.28, and 6.13±0.37 respectively, and were not significantly dif ferent. 2） The ruminal concentration of total VFA tended to increase with the tannin content （P = 0. 099）. Molar proportions of acetic acid were lower （P〈0.05, P=0. 074） while those of propionic acid were higher （P〈0. 088） in treatment III than treatments I andIII , and the A/P was lower （P=0. 096）. 3） The ruminal total N, protein N, bacteria-N, NH3-N and urea-N concentration were not significantly （P〉0.05） different. Cellulolytic microbial activity was restrained to a certain extent by 3.03 g CT/kg of DM from O. viciaefolia hay in the diet but amylolytic microbial activity in the rumen was increased.
出处
《草业学报》
CSCD
北大核心
2010年第1期166-172,共7页
Acta Prataculturae Sinica
基金
国家农业科技成果转化资金项目(2008GB2G100324)
甘肃省农业科技成果转化基金项目(0704XCNA008)
中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)
现代农业产业技术体系建设专项资金资助
