摘要
为研究含氮物质在绒山羊体内的分配规律,本研究选择年龄在2~2.5周岁的绒山羊羯羊,采用消化代谢和氚水稀释技术结合的方法测定不同时期绒山羊总氮沉积量、体氮和毛绒氮的分配量。结果表明,用消化代谢和氚水稀释技术可以准确地测定绒山羊的体氮和毛绒氮的分配量,不同时期的体氮和毛绒氮的分配比例有较大差异。在非生绒期和生绒旺盛期,体氮的分配比例分别为75.7%±0.62%和66.6%±2.2%,毛绒氮的分配比例分别为24.3%±0.62%和33.4%±2.2%,表明不同时期绒山羊的生长方向发生变化。同时发现内分泌也随季节发生变化,主要表现为非生绒期PRL（催乳素）和IGF-I（类胰岛素生长因子I）的水平高,MT（褪黑素）的水平低;生绒旺盛期PRL和IGF-I的水平低,MT的水平高。证明了氚水稀释技术在测定动物氮分配中的可行性,揭示了不同时期含氮物质在绒山羊体内分配的变化规律以及内分泌在这些变化中的调节作用,为不同季节对绒山羊采取合适的饲养管理提供了理论依据。
In order to investigate nitrogen partitioning in local cashmere goats,six Inner Mogolia White Cashmere goats between 2 to 2.5 years old were used to determine the nitrogen partitioning in cashmere goats.The total retained nitrogen（TN）in body,distribution of body nitrgen and hair nitrogen were measured by general digestive and metabolism method combined with tritiated water dilution technique.Results showed that the combined methods were ideal for determining body nitrgen（BN）and hair nitrogen（fur nitrogen,FN）of Cashmere goats.There were obvious significance between BN and FN in different seasons.In telogen,BN and FN partitioning was 75.7%±0.62% and 24.3%±0.62%,respectively.Whereas,it changed to 66.6%±2.2% and 33.4%±2.2% in anagen.BN partitioning decreased when the season changed from telogen to anagen,while FN partitioning increased,which indicated that more nitrogen substance was partitioned to body growth in telogen,and more nitrogen substance was distribute to cashmere growth in anagen.These transformation were related to the changing of photoperiod and some hormones,such as melatonin（MT）,prolactin（PRL） and IGF-I.It could be concluded that tritiated water dilution technique can be used to detect body protein content as well as BN,combining general digestive and metabolism experiment,FN partitoning can be determined.BN and FN partitoning varied with the season in cashmere goats because of hormones changing.
出处
《核农学报》
CAS
CSCD
北大核心
2010年第1期187-191,共5页
Journal of Nuclear Agricultural Sciences
基金
河南农业大学博士科研启动基金(30300098)
关键词
绒山羊
同位素
氚水
稀释技术
氮分配
cashmere goats
isotope
tritiated water
dilution technique
nitrogen partitioning
