摘要
美国白蛾是一种重要的检疫害虫,为进行有效地控制,筛选出了一株对其具有高毒力的苏云金芽孢杆菌。HYW-8是从黑龙江伊春市土壤中通过抗生素筛选法分离到的一株形成小菱形晶体的苏云金芽孢杆菌,利用PCR-RFLP对其基因型进行鉴定,并采用浸液法对美国白蛾进行生物活性测定。结果发现,菌株HYW-8含有cry1Ab、cry1E基因和一种未知cry2基因。生物测定结果表明,其对美国白蛾具有很高的杀虫毒力,LC50为7.4×10^5芽孢/mL,低于标准菌株HD-1。因此,HYW-8对防治美国白蛾具有较强的应用潜力。
Hyphantria cunea was an important quarantine pest.In this study,we selected a Bacillus thuringiensis strain with high virulence for the biocontrol of Hyphantria cunea.The strain HYW-8 was isolated from the soil in the city of Yichun by antibiotic method.It produced small diamond-shaped crystals.It was identified that contained cry1A,cry1E gene and an unknown cry2 gene by PCR-RFLP.Bioassay showed that the strain HYW-8 had high insecticidal activity to Hyphantria cunea.The LC50 was 7.4×10~5 spore/mL,which was lower than the standard strain of Bt subsp kurstaki strain HD-1.We suggested that the potential of the strain HYW-8 for used in microbial control of the larvae of Hyphantria cunea.
出处
《中国农学通报》
CSCD
北大核心
2010年第3期242-244,共3页
Chinese Agricultural Science Bulletin
基金
国家863计划项目(2006AA10A212)
国家科技支撑项目(2008BADA5B03)
