摘要
对野生血红铆钉菇子实体分离的菌种进行了菌丝生长观察和一、二、三级种培养基配方、液体配方试验,结果表明:血红铆钉菇菌丝在试管斜面上25℃4～5天长满,在90 mm平皿3天长满,在-18℃冷冻3天后仍能恢复生长。菌丝一级种的最佳配方是玉米粉PDA培养基;二、三级种以棉籽壳或松林土配合发酵玉米芯更有利于菌丝的生长;液体菌种配方表明以PD为主的配方都适宜血红铆钉菇生长,其中以添加玉米粉、酵母、粪草浸出液能使菌丝生长更加浓密、健壮。
Mycelium growth characters of Gomphidius rutilus strains gained from wild fruit bodies by tissue separate were observed.Culture medium formula of the first-degree strains、the second-degree strains、the third-degree strains and the liquid culture strains were screened.The results showed that the mycelium of the Gomphidius rutilus strains fully colonized in the PDA slant in 4-5 days under the temperature 25℃,fully colonized in the diameter 90mm PDA plate in 3 days,and still restored to growth under the temperature -18℃in 3 days.The best medium formula of the first-degree strains was corn flour add PDA medium.The cottonseed hull or the pine forest soil add fermented corncob were more useful for the second-degree and the third-degree strains growth.All kind of PD medium were suitable for the growth of Gomphidius rutilus liquid culture strains.The mycelium were more densely and stronger on the medium add the corn flour,yeast and manure to crop extraction.
出处
《中国农学通报》
CSCD
北大核心
2010年第3期231-233,共3页
Chinese Agricultural Science Bulletin
基金
北京市属高等学校人才强教计划资助项目"蔬菜优质安全生产理论与技术体系创新研究"学术创新团队资助(PHR200907136)
北京市教委项目(KM200810020011)
