摘要
目的通过研究PAR-1对肺巨细胞癌细胞高、低转移细胞株PLA801D和PLA801C[Ca^2＋]i的影响，探讨PAR-1参与调节肺癌细胞转移相关功能的分子机制。方法激光共聚焦显微镜检测PLA801D和PLA801C细胞[Ca^2＋]i；将反义和正义PAR-1分别转染至PLA801D（D-）和PLA801C（C＋）中；用thrombin和TRAP激活PAR-1，观察PAR-1对D-和C＋的[Ca^2＋]i影响。结果PLA801D（荧光强度59．55，下同）和PLA801C（35．46）细胞之间的平均[Ca^2＋]i差异有统计学意义（P〈0．01）。C＋的[Ca^2＋]i（45．77）明显高于其对照组CV（35．46，P〈0．05），D-的[Ca^2＋]i（48．42）明显低于对照组DV（59．55，P〈0．05）。C＋和CV的[Ca^2＋]i分别在加入thrombin 80s和100s后荧光强度分别达峰值48．19±9．84和45．64±9．87（P〈0．05）；二者均在加入TRAP60s后达峰值，分别为111．31±25．00和52．93±11．21（P〈0．05）。D-和DV的[Ca^2＋]i在加入thrombin 60s后达峰值，分别为40．71±5．89和61．07±21．36（P〈0．05），二者均在加入TRAP40s后达峰值，分别为84．98±11．23和102．58±21．48（P〈0．05）。结论PLA801D和PLA801C的转移潜能可能与其细胞的[Ca^2＋]i有关。激活PAR-1能够上调PLA801D和PLA801C的细胞内Ca^2＋信号。PAR-1促进肺巨细胞癌转移的机制与上调细胞内Ca^2＋有关。
Objectives To investigate molecular mechanisms of PAR-1 regulation on intracellular Ca^2＋ mobilization in lung giant cell carcinoma cells in vitro and its involvement in tumor metastasis. Methods Free intracellular Ca^2＋ （[ Ca^2＋ ] i ） was measured in lung giant cell carcinoma PLA801C and PLA801D cells by confocal microscopy. Sense and anti-sense PAR-1 expression vectors were transfected into PLA801C （C ＋ ）and PLA801D（ D - ） cells, respectively. The effects of PAR-1 expression were investigated by thrombin and TRAP-induced mobilization of [ Ca^2＋ ] i in the C ＋ and D - cells. Results There were significant differences of the mean values of [ Ca^2＋] i between PLAS01D （59.55） and PLA801C cells （ 35.46, P 〈 0.01 ）. The mean [ Ca^2＋ ] i of C ＋ ceils （45.77） was significantly higher than that of its control CV ceils （35.46, P 〈 0. 05 ）, and the mean [ Ca^2＋ ] i of D - cells （48.42） was significantly lower than that of its control DV cells（59. 55,P 〈0. 05）. The peaks of [ Ca^2＋ ] i of C ＋ and CV ceils were 48. 19 ± 9. 84 and 45.64 ± 9.87 （ P 〈 0. 05 ） respectively at 80 s and 100 s after thrombin treatment, but were 111.31 ± 25.00 and 52. 93 ± 11.21 （ P 〈 0. 05 ） respectively at 60 s after TRAP treatment. The peaks of [ Ca^2＋ ] i of D- and DV cells were 40. 71 ± 5.89 and 61.07± 21.36 （ P 〈 0. 05 ） respectively at 60 s after thrombin treatment,but were 84. 98±11.23 and 102. 58 ±21.48 （ P 〈 0. 05 ） respectively at 40 s after TRAP treatment. Conclusions The high metastatic potential of PLA801D and PLA801C may be related to [ Ca^2＋ ] i of the tumor ceils. PAR-1 may play an important role in the metastasis of lung giant cell carcinoma cells by up-regulating the intracellular Ca^2＋.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2010年第2期100-105,共6页
Chinese Journal of Pathology
