摘要
目的观察共刺激分子B7-H1及其受体PD-1在慢性鼻-鼻窦炎患者鼻窦黏膜的表达及分布，探讨其与慢性鼻-鼻窦炎的关系。方法取慢性鼻-鼻窦炎患者鼻窦病变黏膜28例（11例不伴鼻息肉，17例伴有鼻息肉），另以11例正常鼻窦黏膜作为对照，采用实时荧光定量PCR和Western印迹方法分别检测共刺激分子B7-H1及其受体PD-1 mRNA和蛋白的表达；用免疫荧光技术观察其表达和分布；并用流式细胞术检测鼻息肉组织中阳性细胞的类型。结果慢性鼻-鼻窦炎两组患者鼻窦黏膜B7-H1／PD-1 mRNA表达水平高于正常鼻窦黏膜（P〈0．01），两组患者之间差异无统计学意义（P〉0．05）；其蛋白的表达水平与mRNA表达水平基本一致。B7-H1主要表达于患者的鼻窦黏膜上皮细胞、黏膜上皮下的炎性细胞和部分黏膜下腺体上皮细胞；PD-1主要表达于黏膜上皮下的炎性细胞。流式细胞术的结果证实鼻息肉组织中部分B7-H1与PD-1的阳性细胞分别是B细胞与T细胞。结论共刺激分子B7-H1／PD-1在慢性鼻-鼻窦炎的鼻窦黏膜中表达增加，可能在慢性鼻-鼻窦炎的发病机制中起重要作用。
Objective To explore the expression of B7-H1/PD-1 and the localization of these proteins in human sinus mucosa of chronic rhinosinusitis （CRS）. Methods Twenty-eight patients with CRS （17 with nasal polyps （CRSwNP） and 11 without （CRSsNP）） and 11 control patients were recruited. B7-H1/PD-1 expressions in sinus mucosa among these 3 groups were detected by immunofluorescent staining, real-time quantitative PCR and Western blot. Flow cytometry was also employed to identify the type of B7-H1 or PD-1 positive cells in nasal polyps. Results Compared with normal mucosa, B7-H1/PD-1 mRNA was highly expressed in sinonasal mucosa of CRSwNP and CRSsNP （ P 〈 0. 01 ） . There was no difference between CRSwNP and CRSsNP （ P 〉 0. 05 ）. The protein level of B7-H1/PD-1 was similar to mRNA level. As indicated by immunofluorescent assay, B7-H1 was mainly expressed in sinonasal epithelial cells, submucosal gland cells and inflammatory cells within the subepithelial layer while PD-1 was expressed in inflammatory cells within the subepithelial layer. The B7-H1 or PD-1 positive cells in nasal polyps were partially CD19 ^＋ B cells and CD3 ^＋ T cells respectively. Conclusion B7-H1/PD-1 is up-regulated in sinus mucosa of CRS. B7-H1/PD-1 may play an important role in the pathogenesis of CRS.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2010年第6期390-393,共4页
National Medical Journal of China
关键词
鼻窦炎
鼻息肉
T淋巴细胞
鼻黏膜
Sinusitis
Nasal polyps
T-lymphocytes
Nasal mucosa
