摘要
目的探讨联合荧光定量聚合酶链反应（PCR）与血清半乳甘露聚糖（GM）方法用于诊断血液恶性肿瘤／造血干细胞移植患者侵袭性曲霉病（IA）的价值。方法对80例怀疑侵袭性真菌感染的血液恶性肿瘤／造血干细胞移植患者共计205份血清标本同时进行定量PCR和血清半乳甘露聚糖的检测，根据IA诊断标准，分析定量PCR方法与GM方法的符合率及两者联合用于IA诊断时的意义。结果5例患者确诊IA，20例临床诊断IA，34例拟诊IA，21例除外IA。定量PCR方法与GM符合率为77．5％。当定量PCR和GM同时阴性时，诊断侵袭性曲霉感染阴性预测值可达90．9％；当二者同时阳性时，阳性预测值可达100％。结论定量PCR方法与GM方法具有良好符合率，联合两种方法有助于提高IA诊断的准确性。
Objective To evaluate the role of real-time polymerase chain reaction （real-time PCR） assay combined with serum galactomannan（GM） detection in the diagnosis of invasive aspergillosis（IA） with hematological malignancies or hematopoietic stem cell transplantation. Methods Eighty patients were enrolled. PCR and GM were performed simultaneously on 205 serum samples. The agreement between these two assays and the diagnostic value of the combination were analyzed. Results There were 5 proven IA,20 probable IA,34 possible IA and 21 non-IA. Agreement between real-time PCR and GM was 77. 5%. When both positive,the positive predictive value could reach 100% ;when both negative , the negative predictive value could reach 90. 9%. Conclusion PCR agrees well with GM. When these two assays are combined, invasive aspergillosis can be diagnosed more accurately.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2010年第6期375-378,共4页
National Medical Journal of China
基金
公益性卫生行业科研专项基金（200802027）
首都医学发展基金（2005-1010）
关键词
曲霉病
聚合酶链反应
甘露聚糖类
血液肿瘤
造血干细胞移植
Aspergillosis
Polymerase chain reaction
Mannans
Hematologic neoplasms
Hematopoietic stem cell transplantation
