摘要
采用摇瓶培养法对咪唑乙烟酸降解菌株Z的发酵培养基配方进行了研究;并通过单因子试验确定咪唑乙烟酸降解菌株Z的碳源、氮源及无机盐,同时应用正交试验设计对发酵培养基组分进行优化。结果表明:优化后确定的发酵培养基的配方为:麦麸皮1.5%,米糠2%,豆粕1.5%,酵母粉1.5%,NaC l 0.8%,KH2PO40.01%,MnC l20.03%。该研究结果为大规模发酵生产奠定了基础。
The paper studied on the fermentation medium of imazethapyr degradation bacteria Z by shake- flask experiment. The carbon sources, nitrogen sources and inorganic salt of imazethapyr degradation bacteria Z were determined by single ingredient experiment. The factors of fermentation medium were optimized by orthogonal design. The optimal fermentation medium was composed of wheat bran 1.5% ,rice bran 2% ,soybean meal 1.5% ,yeast 1.5% ,NaCl 0.8% ,KH2PO4 0.01% , MnCl2 0.03% ,and the optimized results is suitablefor large scale fermentation production.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第1期84-87,共4页
Soybean Science
基金
黑龙江省自然科学基金资助项目(C2007-20)
大豆生物学省部共建教育部重点实验室开放基金资助项目(SB08B03)
关键词
咪唑乙烟酸
降解菌
发酵培养基
优化
Imazethapyr
Degrading microorganisms
Fermentation medium
Optimization
