摘要
运用电子定位的方法,利用大豆基因组物理图和遗传图的整合图谱,完成了17个大豆天冬氨酸代谢途径中关键酶基因的定位以及结构分析。结果表明:这些酶基因分别定位在A1、B2、D1a、D1b、D2、F、G、H、J、L和N等11个连锁群上,并获得了相应连锁群区间两侧的标记;在基因结构分析上,AHAS、DHDPS、HK与TS没有内含子,DHAD基因的内含子数目最多,为13个,AK基因有12个内含子。通过定位获得的对应分子标记可以为基因分子辅助育种奠定基础,而基因结构信息能更好的实现基因功能分析。
The nutritive value of soybean mainly focused on the aspartate-derived amino acids, and the content of these amino acids were affected by the key enzymes gene from aspartic acid metabolic pathway. In this research, the integrated map between soybean public genetic maps and physical maps was used, and the key enzyme gene sequences were blasted with soybean genome database by local Blast software. Seventeen key enzyme genes in aspartic acid metabolic pathway of soybean were mapped on soybean genetic map, and gene structures were analyzed firstly. The result indicated that these enzyme genes were mapped on 11 linkages group, including A2, B2, C2, D1b, D2, G, I, L, and M, respectively, and the flanking markers of these genes on the linkage group were obtained. The number of introns was from 0 to 13. There was no intron in AHAS,DHDPS,HK,and TS,otherwise there were 13 introns in DHAD,and 12 introns in AK. The corresponding markers obtained from mapping were the base of molecular assisted selection,while the structure information can make gene function analysis easily.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第1期22-27,共6页
Soybean Science
基金
引进国际先进农业科学技术计划资助项目(2006-G1(A))
国家高技术研究发展计划资助项目(2006AA10Z1F4)
黑龙江省博士后科研启动基金资助项目(LHK-04014)
关键词
大豆
天冬氨酸
关键酶基因
电子定位
基因结构
Soybean
Aspartic acid
Key enzyme genes
Electronic mapping
Gene structure
