摘要
[目的]从DNA分子水平上对油棕新品种进行遗传多样性的分析,为这些新选育油棕种质的开发利用提供分子水平的理论依据。[方法]应用ISSR技术对海南和云南的22份油棕新品种进行遗传多样性分析。[结果]用23个引物共扩增得到201条扩增条带,其中多态性条带占41.8%。聚类分析可将22个品种明显的划分为2大类:采自海南的R1~R12新种质材料与采自云南的V20新种质材料聚为一类;采自云南的其余新种质材料聚为一类。[结论]海南与云南新选育的油棕种质材料可能来源于不同的国家或地区。
[Objective]The study aimed to analyze the genetic diversity of new oil palm germplasm from DNA molecular level and provide significant theoretical basis for development and utilization of new oil palm germplasm.[Method]The genetic diversity of 22 new oil palm germplasm from Hainan and Yunnan were analyzed by using ISSR technique.[Result]A total number of 201 bands were generated by using 23 ISSR primers for 22 oil palm varieties,among which 41.8% of the total bands was polymorphic.The cluster analysis could divide these 22 oil palm varieties into two groups:group I including new varieties,R1-R12 from Hainan and V20 from Yunnan;group Ⅱ including new varieties,V13-V22,except V20,from Yunnan.[Conclusion]New breeding oil palm germplasm in Hainan and Yunnan may come from different countries or regions.
出处
《安徽农业科学》
CAS
北大核心
2010年第4期2202-2203,2205,共3页
Journal of Anhui Agricultural Sciences