摘要
[目的]利用4种发根农杆菌R1,R1601,1025,1000诱导药用植物罗勒产生毛状根,建立毛状根培养体系。[方法]利用共培养法研究不同外植体、菌株、预培养时间、感染时间以及外源激素等对罗勒毛状根诱导率的影响。[结果]利用发根农杆菌1025,预培养2d的叶片为转化材料,感染6～8min,加入0.1mg/LNAA的诱导率最高。经过基本培养基的筛选和毛状根生长动力学的考察,确立罗勒毛状根在1/2MS培养基中的最佳继代时间为21d。[结论]罗勒毛状根离体培养的建立,为进一步进行药用活性成分的工业化生产奠定了基础。
[Objective]Hairy root of Ocimum basilicum were induced by the infection of four kinds of Agrobacterium rhizogenes strains R1,R1601,1025 and 1000 and an in vitro culture system of the hairy roots was established.[Method]Hairy roots were induced by co-culture.Effects of explants,Agrobacterium rhizogenes,preculture time,infecting time and phytohormone on the induction rate were studied.[Result] The highest induction rate was obtained from blade leaf with 2 d preculture which were induced by 1025 for 6-8 min.The transformation rate could be raised by culture media with 0.1 mg/L NAA.Through the screen of basic media and the research of growth curve of hairy roots,the optimum inoculum time was selected in 21 days or so in 1/2MS medium.[Conclusion]Establishment of hairy root culture of Ocimum basilicum provided a foundation for the industrial production of active drug component.
出处
《安徽农业科学》
CAS
北大核心
2010年第4期1727-1730,共4页
Journal of Anhui Agricultural Sciences
关键词
罗勒
发根农杆菌
毛状根
Ocimum basilicum L.
Agrobacterium rhizogenes
Hairy roots
