摘要
目的研究脑出血后的脑水肿形成与水通道蛋白4(AQP4)表达及Fe2+含量的关系,以及逐瘀安脑丸的干预作用。方法采用自体不凝血注入法制作大鼠脑出血模型,采用干湿重法、免疫组织化学染色法,测定脑含水量、AQP4蛋白的表达。结果与对照组相比,脑出血组、尼莫地平组、逐瘀安脑丸组均从脑出血后6h开始出现脑水肿(P<0.05),3 d时达到高峰(P<0.01),且逐瘀安脑丸组、尼莫地平组脑水肿低于脑出血组(P<0.05);AQP4表达同样在3 d时达到高峰(P<0.05)。在各时间点脑水肿程度与AQP4表达的变化呈显著正相关,6 h时(r=0.920,P<0.05),1 d时(r=0.952,P<0.05),3 d时(r=0.993,P<0.01),7 d时(r=0.947,P<0.05)。对照组铁离子含量从6 h即开始上升,3 d时达到高峰,之后缓慢下降,7 d时仍高于正常(P<0.05);尼莫地平组各时间点脑铁离子含量均低于对照组(P<0.05),逐瘀安脑丸大剂量组3 d和7 d时间点上脑铁离子含量均低于尼莫地平组(P<0.05)。结论脑出血后AQP4、Fe2+表达明显增加,提示AQP4、Fe2+参与了脑水肿的发生发展过程,而逐瘀安脑丸可能通过降低Fe2+的含量以下调AQP4的表达,减轻脑出血后脑水肿的形成。
Objective To study the relationship between the cerebral edema after cerebral hemorrhage and AQP4, Fe^2＋ expression, and the interference effect of Zhuyu Annao Pill. Methods The ICH model was established by stereotaxic injection of auto - nonanticoagulated artery blood into the caudate nucleus, AQP4 were detected expression by innumumohistochemistry and cerebral edema（ cerebral water contents）. Results Compared with control group, cerebral edema appeared in ICH group, Zhuyu Annao Pill group and nimodip group 6 h after cerebral hemorrhage （P 〈 0.05 ） , and cerebral edema in Zhuyu Annao Pill group, that in nimodipine group was lower than that of cerebral hemorrhage group （ P 〈 0.05 ）. AQP4 expression reached the highest level at 3 d （ P 〈 0.05 ）. There was significant possitive correlation between the extent of brain edema and changes in AQP4 expression at each time, 6 h （r =0. 920, P 〈0.05）, 1 d, when （r =0. 952,P 〈0.05）, 3 d （r =0.993, P 〈 0.01）, 7 d （r =0. 947,P〈0.05）. In control group, iron ion content began to increase from 6 h, 3d peaked on the 3rd day, followed by a slow decline, it was still higher than normal on the 7 th day （ P 〈 0.05 ） ; brain iron ion content in nimodipine group at different time points were lower than that in control group （P 〈 0.05 ）. Brain iron ion content in high dose group was lower than that of nimodopine group on the 3 rd and 7 th day （ P 〈 0. 05 ）. Conclusion AQP4 expression increases significantly after cerebral hemorrhage, which suggests that APQ4 and Fe^2 ＋ involved in pathogenesis and development of cerebral edema. Zhuyu Annao Pill, can inhibit cerebral edema by reducing Fe^2 ＋ content and down - regutating the expression of AQP4.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第2期383-385,共3页
Lishizhen Medicine and Materia Medica Research
基金
广西科技厅回国基金资助项目(No.0575017)
广西中医学院研究生导师课题(No.Y2007045)
