摘要
目的探讨原花青素(procyanidin,PC)对脑缺血再灌注损伤大鼠Caspase-3和Caspase-9活性的影响。方法将大鼠随机分为假手术组、缺血再灌注组、PC低剂量治疗组和PC高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。采用四肽酶底物法检测脑缺血90 min再灌注24 h大鼠Caspase-3和Caspase-9活性,进行神经症状评分,2,3,5-三苯基氯化四氮唑(TTC)染色和HE染色观察脑梗塞体积及病理形态学变化。结果脑缺血再灌注24 h,缺血再灌注组Caspase-3和Caspase-9活性增加,与假手术组比较,差异具有显著性(均P<0.05);与缺血再灌注组比较,PC高、低剂量治疗组均显著减少Caspase-3和Caspase-9活性,高、低剂量组之间差异亦具有显著性(P<0.05)。PC高、低剂量治疗组神经功能缺失评分较缺血再灌注组降低(P<0.05)。PC高、低剂量治疗组脑梗死体积较缺血再灌注组减小,高、低剂量组之间差异具有显著性(P<0.05)。PC高、低剂量治疗组脑组织缺血损伤病理学改变明显轻于缺血再灌注组,PC高剂量治疗组缺血改变亦轻于低剂量治疗组。结论PC对缺血再灌注脑损伤具有保护作用,其机制可能与减少Caspase-3和Caspase-9活性,抗凋亡有关。
Objective To study the effect of proeyanidin on the activities of Caspase - 3 and Caspase - 9 after cerebral ischemie reperfusion（I/R） injury in rats. Methods In this experiment, rats were randomly divided into 4 groups：sham operated group, I/ R group,low procyanidin treated group and high procyanidin treated group. The focal middle cerebral artery occlusion （ MCAO ） model was made by suture - occluded method. After 90min MCAO following 24h repeffusion, we investigated the activities of Caspase - 3 and Caspase - 9 with using cleavage of the fluorescence substrate AC - DEVD - AMC and AC - L EHD - AFC respectively. The neurological score was investigated. TTC and HE staining were also investigated. Results Compared with sham operated group , the activities of Caspase - 3 and Caspase - 9 were increased at 24h reperfusion in the ischemic territory （ P 〈 0.05）. Compared with I/R group, low and high dose procyanidin dose - dependently reduced the activities of Caspase - 3 and Caspase- 9 dose- dependently （P 〈 0.05 ）. The neurological score, and cerebral infarction volume alleviated the pothological changes of brain tissue. Conclusion Procyanidin plays protective effect on cerebral ischemia injury by decreasing the activities of Caspase -3 and Caspase -9, and then decreasing neuronal apoptosis.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第2期317-319,共3页
Lishizhen Medicine and Materia Medica Research
基金
广西壮族自治区自然科学基金(No.0848015)
