摘要
以海洋烟曲霉菌为出发菌,在适宜的发酵条件下进行培养,提取代谢产物并检测其对白色念珠菌抑菌性.分别以硫酸铵、石油醚、乙酸乙酯为萃取剂,研究硫酸铵质量分数和有机溶剂的pH值对海洋烟曲霉菌代谢活性物质萃取效果的影响.结果表明,粗酶液的适宜萃取条件:萃取剂为石油醚,pH值为6,粗酶液与有机溶剂的体积比为1∶1.采用质量分数为70%的硫酸铵沉淀,萃取活性物质的酶活性最大为16.0μkat.L-1,比原粗酶液的酶活(7.9μkat.L-1)提高1倍;而采用石油醚为萃取剂,所萃取活性物质的酶活性最大为20.5μkat.L-1,比原粗酶液的酶活提高2.58倍,且抑菌性能最好,抑菌率达60%左右.
Marine Aspergillus furnigatus were cultured under a condition suitable for fermatation, and metabolic active substances were then extracted to test their inhibiting to Candida albicans. Using ammonium sulfate, petroleum ether, ethyl acetate as extraetant respectively, the influence to extraction active substance of marine Aspergillus fumigatus with organic solvent and different pH were studied, and extraction condition were investigated. The experimental results indicate that the suitable condition of crude enzyme was petroleum ether as extractant, pH= 6, the volume rate of crude fermented liquors with organic solvent is 1 ： 1. The enzyme active of extrating substance is about 16.0 μkat · L^-1 when using 70% ammonium sulfat extractant, one times higher than the activity of crude fermented liquors （about 7.9μkat · L^-1 ）. While, the enzyme active of extrating substance is about 20. 5 μkat · L^-1 when using petroleum ether extractant, it increase 2. 58 times to the activity of crude fermented liquors and having the best inhibiting, the inhibiting is about 60% to Candida albicans.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2010年第2期192-195,共4页
Journal of Huaqiao University(Natural Science)
基金
福建省科技计划重点项目(2007T0010)
福建省自然科学基金资助项目(E0640003)
关键词
烟曲霉菌
脱乙酰酶
活性物质
萃取
抑菌性
Aspergillusfumigatus
deacetylase
active substance
extraction
inhibiting
