摘要
目的:探讨胸腺基质淋巴生成素(thymic stromal lymphopoietin,TSLP)在肺癌组织中的表达及其与临床指标以及局部调节性T细胞(Regulatory T cells,Tregs)数量的相关性。方法:分别采用Q-RT-PCR和免疫组织化学染色方法检测TSLP在不同病变类型的肺组织中的表达,分析TSLP在不同病变肺组织中的表达差异;并运用免疫组化方法检测TSLP蛋白在不同病理类型的肺癌组织中的表达,分析TSLP表达与临床病理特征之间的相关性;采用免疫组化法检测肺癌组织中的Tregs细胞,分析TSLP表达与肿瘤局部Tregs细胞数量之间的关系。结果:TSLP基因在肺癌组织、癌旁组织、非肿瘤肺上皮均表达阳性,且表达差异无统计学意义;TSLP蛋白表达于胞浆中,其在肺癌组织中的阳性表达率(69.57%)显著高于肺的良性病变(13.33%)和非肿瘤肺上皮(30.00%),且TSLP表达与肿瘤大小和淋巴结转移有关;TSLP表达阳性的患者其肿瘤局部浸润的调节性T细胞数量明显高于TSLP阴性组(P<0.05)。结论:TSLP蛋白在肺癌组织中表达增加,且与肿瘤局部调节性T细胞数量增多有关,这提示TSLP可能是通过诱导Tregs增加在肺癌免疫耐受中发挥作用。
Objective： To investigate the expression of TSLP in human lung cancer tissue and the correlation between TSLP expression and number of regulatory T cells （Tregs）. Methods： The expression of TSLP mRNA and protein was detected in different pathological lesions of the lung by Q-RT-PCR and immunohistochemistry. Immunohistochemistry was used to detect Foxp3＋ Tregs. The correlation of TSLP with the number of Tregs was analyzed. Results： TSLP gene was expressed in tumor tissues （n=37）, latero-tumor tissues （n= 29） and non-tumor lung tissues （n=24）, without statistical difference （P=0.148）. TSLP protein was expressed in the cytoplasm and was observeed in 69.57% of tumor tissues, 13.33% of benign lesions and 30.00% of non-tumor lung tissues, with a significant difference （P〈0.05）. The expression of TSLP protein was correlated with tumor size （P=0.000） and lymph node metastasis （P=0.018）. The number of Tregs in TSLP positive group was more than that in TSLP negative group （P〈0.05）. Conclusion： The expression of TSLP in lung tumor tissues is increased and is correlated with the number of Tregs, indicating that TSLP could induce Treg toplay an important role in tumor immunotolerance.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2010年第3期126-130,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金(编号:30872986)
天津市高等学校科技发展基金资助(编号:20060110)~~
