摘要
目的探讨磁刺激对脊髓内源性神经干细胞（NSCs）增殖分化的作用。方法将46只Wistar大鼠分为正常组、对照组和治疗组，对照组和治疗组采用改良的Allen重物坠落法造成大鼠脊髓损伤（SCI）模型，治疗组于SCI后24h开始，每天给予脉冲磁刺激，频率为0．5Hz，强度为1．44T，每次30个脉冲，每天1次，连续7d。应用BBB运动功能评分评价磁刺激治疗后大鼠运动功能的改善，采用免疫组织荧光化学技术检测脊髓神经上皮干细胞巢蛋白的表达，采用免疫荧光三标技术结合激光共聚焦显微镜检测微管相关蛋2（MAP2）／巢蛋白／4，6-二乙酰基．2-苯基吲哚（DAPI）的表达。结果SCI后，治疗组大鼠运动功能改善明显，治疗组脊髓中央管室管膜、室管膜下区、损伤病灶周围和血管周围巢蛋白阳性细胞数较对照组多（P〈0．05），且尽管总体细胞数量有限，在上述部位的MAP2／巢蛋白双标阳性细胞数多于对照组。结论磁刺激可以促进脊髓内源性NSCs的激活，并有可能诱导其向神经元细胞分化，该诱导分化作用是否在神经再生方面具有实质性意义值得进一步深入研究。
Objective To investigate the effects of magnetic stimulation （MS） on the proliferation and differentiation of endogenous neural stem cells （NSCs）/progenitor cells after spinal cord injury （SCI） in rats. Methods Forty-six Wistar rats were used, of which 40 were used to make an animal model of spinal cord injury （SCI） by administering a 10 g × 12.5 cm impact at the T8 level. The other 6 served as the normal controls. The SCI model rats were evenly divided into a magnetic stimulation （MS） group （n = 20） and a control group （n = 20）. The rats in the MS group received 0.5 Hz and 1.44 T magnetic stimulation 24 h post injury, then 30 pulses per day for 7 days. The rats in the other groups were not exposed to MS. The scale of Basso, Beatti and Bresnahan （BBB） was used to assess hindlimb neurological function. Rats were sacrificed at the 24th hour, and at the 1 st, 4th and 8th weeks after SCI. The ratio of nestin to microtubule associated protein 2 （MAP2）/nestin in the ceils of the spinal cord was determined by immunofluorescence. Results The BBB scores in the MS group were signifi- cantly higher than those of the control group at 1, 4 and 8 weeks post SCI. Nestin and the MAP2/nestin ratios were mild in the normal spinal cords, but increased after SCI. They were higher in the MS group than that in the control groups at all time points. Conclusions MS can promote nestin expression in the spinal cord after SCI and facilitate neural differentiation.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2010年第1期2-5,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
磁刺激
脊髓损伤
神经干细胞
微管相关蛋2
巢蛋白
Magnetic stimulation
Spinal cord injury
Neural stem cells
Microtubule associated protein 2
Nestin
